Expression of hepatic 3β-hydroxysteroid dehydrogenase and sulfotransferase 2A1 in entire and castrated male pigs

被引:13
作者
Rasmussen, Martin Kroyer [1 ]
Brunius, Carl [2 ]
Ekstrand, Bo [1 ]
Zamaratskaia, Galia [2 ]
机构
[1] Aarhus Univ, Dept Food Sci, DK-8830 Tjele, Denmark
[2] Swedish Univ Agr Sci, Dept Food Sci, Bioctr, Uppsala, Sweden
关键词
Castration; Gene regulation; Protein expression; Boar taint; Androstenone; DEHYDROEPIANDROSTERONE SULFOTRANSFERASE; BOAR TAINT; 16-ANDROSTENE STEROIDS; GENE-EXPRESSION; ESTRONE SULFATE; ANDROSTENONE; METABOLISM; SULFOCONJUGATION; TESTOSTERONE; SULT2A1;
D O I
10.1007/s11033-012-1637-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present study investigated the effect of surgical (SC) and immunological castration on the steroid metabolizing enzymes 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and sulfotransferase 2A1 (SULT2A1) in male pigs. Thirty-two male pigs were divided in four groups; in one group the pigs were SC before the age of 7 days, two groups were injected with Improvac(A (R)) a vaccine against gonadotropin releasing hormone (immunological castration), while the pigs in the last group remained entire males (EMs). Immunological castration was in one group performed by vaccine injection at ages 11 and 14 weeks, while the other group received injections at ages 17 and 21 weeks. Plasma, adipose and liver tissue were collected at the time of slaughter. Plasma was analyzed for concentrations of testosterone and oestradiol. The adipose tissue was analyzed for the concentration of androstenone, while the liver tissue was analyzed for mRNA and protein expression of 3 beta-HSD and SULT2A1. Independent of method, all castrated pigs showed greater mRNA and protein expression of 3 beta-HSD and lower levels of all steroids in plasma compared with EMs. Moreover, there was a strong correlation between mRNA and protein expression of 3 beta-HSD and steroid levels. The same was not valid for expression of SULT2A1. It is concluded that steroid levels can increase expression of the steroid metabolizing enzyme 3 beta-HSD and thereby influence steroid metabolism, e.g. of androstenone.
引用
收藏
页码:7927 / 7932
页数:6
相关论文
共 50 条
[41]   Biological Roles of Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1), HSD11B2, and Glucocorticoid Receptor (NR3C1) in Sheep Conceptus Elongation [J].
Brooks, Kelsey ;
Burns, Gregory ;
Spencer, Thomas E. .
BIOLOGY OF REPRODUCTION, 2015, 93 (02)
[42]   Liver-specific G0/G1 switch gene 2 (G0s2) expression promotes hepatic insulin resistance by exacerbating hepatic steatosis in male Wistar rats [J].
Sugaya, Yoshiyuki ;
Satoh, Hiroaki .
JOURNAL OF DIABETES, 2017, 9 (08) :754-763
[43]   Augmenting effects of gestational arsenite exposure of C3H mice on the hepatic tumors of the F2 male offspring via the F1 male offspring [J].
Nohara, Keiko ;
Okamura, Kazuyuki ;
Suzuki, Takehiro ;
Murai, Hikari ;
Ito, Takaaki ;
Shinjo, Keiko ;
Takumi, Shota ;
Michikawa, Takehiro ;
Kondo, Yutaka ;
Hata, Kenichiro .
JOURNAL OF APPLIED TOXICOLOGY, 2016, 36 (01) :105-112
[44]   Supranutritional selenium intake from enriched milk casein impairs hepatic insulin sensitivity via attenuated IRS/PI3K/AKT signaling and decreased PGC-1α expression in male Sprague-Dawley rats [J].
Stahel, Priska ;
Kim, Julie J. ;
Cieslar, Scott R. L. ;
Warrington, Jenny M. ;
Xiao, Changting ;
Cant, John P. .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2017, 41 :142-150
[45]   Influence of dietary zinc deficiency during development on hepatic CYP2C11, CYP2C12, CYP3A2, CYP3A9, and CYP3A18 expression in postpubertal male rats [J].
Xu, ZM ;
Kawai, M ;
Bandiera, SM ;
Chang, TKH .
BIOCHEMICAL PHARMACOLOGY, 2001, 62 (09) :1283-1291
[46]   Biosynthesis and Degradation of Canine Placental Prostaglandins: Prepartum Changes in Expression and Function of Prostaglandin F2alpha-Synthase (PGFS, AKR1C3) and 15-Hydroxyprostaglandin Dehydrogenase (HPGD) [J].
Gram, Aykut ;
Buechler, Urs ;
Boos, Alois ;
Hoffmann, Bernd ;
Kowalewski, Mariusz P. .
BIOLOGY OF REPRODUCTION, 2013, 89 (01)
[47]   Faster clearance of mirodenafil in rats with acute renal failure induced by uranyl nitrate: contribution of increased protein expression of hepatic CYP3A1 and intestinal CYP1A1 and 3A1/2 [J].
Choi, Young H. ;
Lee, Young S. ;
Kim, Tae K. ;
Lee, Bong-Y. ;
Lee, Myung G. .
JOURNAL OF PHARMACY AND PHARMACOLOGY, 2009, 61 (10) :1325-1332
[48]   A novel mechanism underlying alcohol dehydrogenase expression: hsa-miR-148a-3p promotes ADH4 expression via an AGO1-dependent manner in control and ethanol-exposed hepatic cells [J].
Luo, Jiao ;
Hou, Yufei ;
Ma, Wanli ;
Xie, Mengyue ;
Jin, Yuan ;
Xu, Lin ;
Li, Chuanhai ;
Wang, Ying ;
Chen, Jing ;
Chen, Wendi ;
Zheng, Yuxin ;
Yu, Dianke .
BIOCHEMICAL PHARMACOLOGY, 2021, 189
[49]   Expression of NR3C1, INSR and SLC2A4 genes in skeletal muscles and CBG in liver depends on age and breed of pigs [J].
Szczepankiewicz, Dawid ;
Skrzypski, Marek ;
Pruszynska-Oszmalek, Ewa ;
Sadkowski, Slawomir ;
Sassek, Maciej ;
Wojciechowicz, Tatiana ;
Kolodziejski, Pawel Antoni ;
Kaczmarek, Przemyslaw ;
Szydlowski, Maciej ;
Mackowiak, Pawel ;
Nowak, Krzysztof Wojciech .
CZECH JOURNAL OF ANIMAL SCIENCE, 2019, 64 (08) :343-351
[50]   The study of effect of resistance training on plasma S1P level and gene expression of S1P1,2,3 receptors in male Wistar rat [J].
Banitalebi, E. ;
Gharakhanlou, R. ;
Ghatrehsamani, K. ;
Parnow, A. H. ;
Teimori, H. ;
Amoli, M. Mohammad .
MINERVA ENDOCRINOLOGICA, 2013, 38 (04) :395-400