Inhibition of Nitric Oxide Mediated Protein Nitration Therapeutic Implications in Experimental Radiculopathy

被引:4
|
作者
Lee, Seong Jae [1 ]
Kim, Tae Uk [1 ]
Park, Jeong-Soo [2 ,3 ]
Ra, Jong Yun [1 ]
机构
[1] Dankook Univ, Coll Med, Dept Rehabil Med, Cheonan, Choongnam, South Korea
[2] Dankook Univ, Coll Med, Dept Biochem, Cheonan, Choongnam, South Korea
[3] Dankook Univ, Inst Tissue Regenerat Engn ITREN, Cheonan, Choongnam, South Korea
关键词
radiculopathy; nitric oxide; protein; nitration; nitrotyrosine; aminoguanidine; TYROSINE NITRATION; HISTOLOGIC-CHANGES; RADICULAR PAIN; NERVE; ISCHEMIA; MODEL; IRRITATION; EXPRESSION; MARKERS; INJURY;
D O I
10.1097/BRS.0b013e3182a085d9
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Study Design. Experimental animal study. Objective. This study investigated whether nitric oxide (NO) mediated protein nitration is involved in the pathogenesis of radiculopathy and whether the symptoms can be relieved by its suppression. Summary of Background Data. It has been reported that nitration of protein mediated by NO is involved in the degenerative neurological disorders, but its involvement is not clear in the radiculopathy. Methods. Two kinds of rat models of radiculopathy were used. Radiculopathy was induced either by ligation of spinal nerve roots or transplantation of autologous nucleus pulposus. In separate groups of rats, aminoguanidine, a potent nitric oxide synthetase inhibitor, was administered just before induction of radiculopathy, to suppress NO production and resultant nitration of protein. Sensation of the hind limb was evaluated by plantar stimulation test, and motor weakness was assessed by observation of gait pattern. Nitrotyrosine, product of protein nitration, was assayed quantitatively by Western immunoblotting. Results. Mechanical allodynia was observed in both compression and nucleus pulposus groups, but motor weakness was observed only in the compression group. Preoperative administration of aminoguanidine attenuated mechanical allodynia and motor weakness. Optical densities of nitrotyrosine bands increased signifi cantly in radiculopathy groups, but they were lowered by administration of aminoguanidine. Conclusion. NO mediated protein nitration contributes to the development of both types of radiculopathies. Suppression of NO production can decrease protein nitration and relieve neural dysfunctions of radiculopathy.
引用
收藏
页码:1749 / 1753
页数:5
相关论文
共 50 条
  • [31] Effects of nitric oxide synthase inhibition on intestinal damage in rats with experimental necrotizing enterocolitis
    Ciftçi, I
    Dilsiz, A
    Aktan, TM
    Gürbilek, M
    Duman, S
    EUROPEAN JOURNAL OF PEDIATRIC SURGERY, 2004, 14 (06) : 398 - 403
  • [32] Lack of involvement of nitric oxide in the macrophage-mediated inhibition of spleen cell proliferation during experimental cryptococcosis
    Rossi, GR
    Cervi, LA
    Sastre, DA
    Masih, DT
    CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1998, 86 (01): : 16 - 26
  • [33] Differential inhibition of Maillard protein fluorescence by nitric oxide donors
    Suárez, G
    Wang, XH
    NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 1998, 2 (06): : 475 - 480
  • [34] SYMPATHETICALLY MEDIATED HYPERTENSION CAUSED BY CHRONIC INHIBITION OF NITRIC-OXIDE
    SANDER, M
    HANSEN, PG
    VICTOR, RG
    HYPERTENSION, 1995, 26 (04) : 691 - 695
  • [35] Aspects, mechanism, and biological relevance of mitochondrial protein nitration sustained by mitochondrial nitric oxide synthase
    Elfering, SL
    Haynes, VL
    Traaseth, NJ
    Ettl, A
    Giulivi, C
    AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2004, 286 (01): : H22 - H29
  • [36] Nitric oxide mediated inhibition of contractile activity in the human uterine cervix
    Ekerhovd, E
    Brannstrom, M
    Delbro, D
    Norstrom, A
    MOLECULAR HUMAN REPRODUCTION, 1998, 4 (09) : 915 - 920
  • [37] Sildenafil potentiates nitric oxide mediated inhibition of human platelet aggregation
    Gudmundsdóttir, IJ
    McRobbie, SJ
    Robinson, SD
    Newby, DE
    Megson, IL
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 337 (01) : 382 - 385
  • [38] Nitric oxide system and protein nitration are modified by an acute hypobaric hypoxia in the adult rat hippocampus
    Encinas, JM
    Serrano, J
    Bentura, ML
    Castro-Blanco, S
    Fernández, P
    Rodrigo, J
    JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 2003, 62 (08) : 863 - 877
  • [39] Implications of microvascular dysfunction and nitric oxide mediated inflammation in severe COVID-19 infection
    Jani, Vinay P.
    Munoz, Carlos J.
    Govender, Krianthan
    Williams, Alexander T.
    Cabrales, Pedro
    AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 2022, 364 (03) : 251 - 256
  • [40] Effects of oxygen and glucose deprivation on the expression and distribution of neuronal and inducible nitric oxide synthases and on protein nitration in rat cerebral cortex
    Alonso, D
    Serrano, J
    Rodríguez, I
    Ruíz-Cabello, J
    Fernández, AP
    Encinas, JM
    Castro-Blanco, S
    Bentura, ML
    Santacana, M
    Richart, A
    Fernández-Vizarra, P
    Uttenthal, LO
    Rodrigo, J
    JOURNAL OF COMPARATIVE NEUROLOGY, 2002, 443 (02) : 183 - 200