Regulatory associations between the metabolism of sulfur-containing amino acids and xanthan biosynthesis in Xanthomonas campestris pv. campestris B100

被引：5

作者：

Schulte, Fabian ^{[1
]}

Lessmeier, Lennart ^{[2
]}

Voss, Julia ^{[1
]}

Ortseifen, Vera ^{[1
]}

Vorhoelter, Frank-Joerg ^{[1
,3
]}

Niehaus, Karsten ^{[1
]}

机构：

[1] Univ Bielefeld, Fac Biol, Ctr Biotechnol, Dept Proteome & Metabolome Res Bio27, Univ Str 25, D-33615 Bielefeld, Germany

[2] Univ Bielefeld, Fac Biol, Ctr Biotechnol, Chair Genet Prokaryotes, Univ Str 25, D-33615 Bielefeld, Germany

[3] MVZ Dr Eberhard & Partner, Brauhausstr 4, D-44134 Dortmund, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2019年 / 366卷 / 02期

关键词：

Xanthomonadaceae; bacterial polysaccharide production; transcriptional regulation; phytopathogen; CELL-CELL COMMUNICATION; DNA REGION; IDENTIFICATION; PROTEIN; GUM; METHIONINE; GENOME; SIGNAL; SYSTEM; TRANSCRIPTION;

D O I：

10.1093/femsle/fnz005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gamma-proteobacterium Xanthomonas campestris pv. campestris (Xcc) B100 synthesizes the exopolysaccharide xanthan, a commercially relevant thickening agent produced commonly by industrial scale fermentation. This work was inspired by the observation that methionine is an inhibitor of xanthan formation in growth experiments. Therefore, the global effects of methionine supplementation were characterized through cultivation experiments, genome-wide microarray hybridizations and qRT-PCR. Specific pull down of DNA-binding proteins by using the intergenic regions upstream of xanA, gumB and gumD led to the identification of six transcriptional regulators, among them the LysR-family transcriptional regulator CysB. An insertion mutant of this gene was analyzed by growth experiments, microarray experiments and qRT-PCR. Based on our experimental data, we developed a model that describes the methionine-dependent co-regulation of xanthan and sulfur-containing compounds in Xanthomonas. These data substantially contribute to better understand the impact of methionine as a compound in xanthan production media used in industrial fermentations.

引用

页数：11