Antitumorigenic Effect of Dietary Natural Compounds via Lipid Metabolism Modulation in ApcMin/+ Mice

Aim: The aim of the present study was to evaluate the effects of a diet supplementation with either olive oil, or n-3 or n-6-polyunsaturated Fatty acids (PUFAs) on tumour development and gene expression for lipogenic enzymes in Apc(Min/+) mice. Materials and Methods: In the control group, the mice received a standard diet, the 00 group was fed on a diet with 12% olive oil, the OM-3 group with 12% salmon fish rich in n-3 PUFAs, the OM-6 group with 12% oenothera oil rich in n-6 PUFAs. Gene expression of lipogenic enzymes was evaluated by real-time reverse transcription polymerase chain reaction. Results: All mice in the treated groups presented a reduction in total intestinal polyp number and load, which was particularly marked in the OM-3 group. Treated mice showed an induction of low density lipoprotein receptor gene expression and a significant reduction of expression of lipogenic gene. Conclusion: Our data provide new insights into the mechanism of cell growth inhibition and apoptotic regulation by dietary olive oil and PUFAs in Apc(Min/+) mice.