The FDAM method: Determination of carboxyl profiles in cellulosic materials by combining group-selective fluorescence labeling with GPC

被引：86
作者：
Bohrn, R.
Potthast, A.
Schiehser, S.
Rosenau, T.
Sixta, H.
Kosma, P.
机构：
[1] Univ Nat Resources & Appl Life Sci Vienna, Dept Chem, A-1190 Vienna, Austria
[2] Univ Nat Resources & Appl Life Sci Vienna, Christian Doppler Lab Heterogene Katalyse, A-1190 Vienna, Austria
[3] Lenzing AG, R&D, A-4860 Lenzing, Austria
来源：
BIOMACROMOLECULES | 2006年 / 7卷 / 06期
关键词：
D O I：
10.1021/bm060039h
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel method for accurate determination of the carboxyl content in cellulosic materials by fluorescence labeling with 9H-fluoren-2-yl-diazomethane (FDAM) has been developed. The procedure can readily be implemented into a GPC system with RI and MALLS detectors, requiring additional fluorescence detection. The labeling conditions were optimized by means of sugar acid model compounds and were transferred to the cellulose case. Kinetics of the labeling and the influence of reaction parameters were comprehensively studied. For the first time, carboxyl profiles of cellulosics, i.e., the carboxyl content relative to the molecular weight distribution, were obtained.
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收藏
页码：1743 / 1750
页数：8
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