Protective effects of camosine, homocarnosine and anserine against peroxyl radical-mediated Cu,Zn-superoxide dismutase modification

被引:70
|
作者
Kang, JH [1 ]
Kim, KS
Choi, SY
Kwon, HY
Won, MH
Kang, TC
机构
[1] Chongju Univ, Dept Genet Engn, Div Nat Sci, Chonju 360764, South Korea
[2] Hallym Univ, Dept Genet Engn, Div Life Sci, Chunchon 200702, South Korea
[3] Hallym Univ, Coll Med, Dept Physiol, Chunchon 200702, South Korea
[4] Hallym Univ, Coll Med, Dept Anat, Chunchon 200702, South Korea
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2002年 / 1570卷 / 02期
关键词
Cu; Zn-superoxide dismutase; fragmentation; peroxyl radical; carnosine;
D O I
10.1016/S0304-4165(02)00158-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Carmosine (beta-alanyl-L-histidine), homocamosine (gamma-amino-butyryl-L-histidine) and anserine (beta-alanyl-1-methyl-L-histidine) have been proposed to act as anti-oxidants in vivo. The protective effects of carnosine and related compounds against the oxidative damage of human Cu,Zn-superoxide dismutase (SOD) by peroxyl radicals generated from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) were studied. The oxidative damage to Cu,Zn-SOD by AAPH-derived radicals led to protein fragmentation, which is associated with the inactivation of enzyme. Camosine, homocamosine and anserine significantly inhibited the fragmentation and inactivation of Cu,Zn-SOD by AAPH. All three compounds also inhibited the release of copper ions from the enzyme and the formation of carbonyl compounds in AAPH-treated Cu,Zn-SOD. These compounds inhibited the fragmentation of other protein without copper ion. The results suggest that carnosine and related compounds act as the copper chelator and peroxyl radical scavenger to protect the protein fragmentation. Oxidation of amino acid residues in Cu,Zn-SOD induced by AAPH were significantly inhibited by carnosine and related compounds. It is proposed that carnosine and related dipeptides might be explored as potential therapeutic agents for pathologies that involve Cu,Zn-SOD modification mediated by peroxyl radicals. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:89 / 96
页数:8
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