MOLECULAR SPECTROSCOPY EVIDENCE OF EFFECTIVE CONSTITUENTS IN THE BINDING OF Angelica sinensis TO BOVINE SERUM ALBUMIN: COMPARATIVE BINDING AND THERMODYNAMIC PROFILE

被引：1

作者：

Wang, Zhenzhong ^{[1
,2
,3
]}

Deng, Xuehua ^{[1
,2
,3
]}

Hou, Jia ^{[4
]}

Fan, Jing ^{[1
,2
,3
]}

机构：

[1] Hubei Univ Med, Coll Pharm, Shiyan 420000, Peoples R China

[2] Key Lab Wudang Characterist Herbal Study Hubei Pr, Shiyan 420000, Peoples R China

[3] Engn Res Ctr Herbal Utilizat Hubei Prov, Shiyan 420000, Peoples R China

[4] Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China

来源：

JOURNAL OF APPLIED SPECTROSCOPY | 2019年 / 85卷 / 06期

关键词：

Angelica sinensis; fluorescence; bovine serum albumin; ferulic acid; butylidenephthalide; senkyunolide I; ACID; COMPONENTS;

D O I：

10.1007/s10812-019-00760-1

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Some bio-effective constituents, such as organic acids and phthalides, from the root of Angelica sinensis can be observed in the blood after oral intake. In this work, two organic acids, ferulic acid and anisic acid, and two phthalides, senkyunolide I and butylidenephthalide, were selected to investigate the interaction with bovine serum albumin systematically, using a fluorescence spectroscopic method under physiological conditions. The Stern-Volmer quenching constant, binding constant, and the corresponding thermodynamic parameters and binding numbers were measured. Synchronous fluorescence and three-dimensional fluorescence spectroscopy were also used. The results provided molecular spectroscopy evidence that some constituents of Angelica sinensis are absorbed and transported into the blood.

引用

页码：1064 / 1070

页数：7