Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases

被引：54

作者：

Balintova, Jana ^{[1
,2
]}

Plucnara, Medard ^{[3
]}

Vidlakova, Pavlina ^{[3
]}

Pohl, Radek ^{[1
,2
]}

Havran, Ludek ^{[3
]}

Fojta, Miroslav ^{[3
,4
]}

Hocek, Michal ^{[1
,2
,5
]}

机构：

[1] Acad Sci Czech Republ, Inst Organ Chem & Biochem, Gilead Sci, CR-16610 Prague 6, Czech Republic

[2] IOCB Res Ctr, Prague 16610 6, Czech Republic

[3] Acad Sci Czech Republ, Inst Biophys, Vvi, CS-61265 Brno, Czech Republic

[4] Masaryk Univ, Cent European Inst Technol, Brno 62500, Czech Republic

[5] Charles Univ Prague, Fac Sci, Dept Organ Chem, Prague 12843 2, Czech Republic

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2013年 / 19卷 / 38期

关键词：

DNA polymerase; electrochemistry; nucleoside triphosphates; sequencing; voltammetry; NUCLEOSIDE TRIPHOSPHATES; POLYMERASE INCORPORATION; UNPROTECTED HALONUCLEOSIDES; ENZYMATIC INCORPORATION; NUCLEOTIDES; DERIVATIVES; WATER; OLIGONUCLEOTIDES; SPECIFICITY; NUCLEOBASE;

D O I：

10.1002/chem.201301868

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. In combination with nitrophenyl and aminophenyl labels, we have successfully developed a three-potential coding of DNA bases and have explored the relevant electrochemical potentials. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.

引用

页码：12720 / 12731

页数：12

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