Molecular beacon structure mediated rolling circle amplification for ultrasensitive electrochemical detection of microRNA based on quantum dots tagging

被引：41

作者：

Wang, Danchen ^{[1
]}

Hu, Lihui ^{[1
]}

Zhou, Huiming ^{[1
]}

Abdel-Halim, E. S. ^{[2
]}

Zhu, Jun-Jie ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China

[2] King Saud Univ, Coll Sci, Dept Chem, Riyadh 11451, Saudi Arabia

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2013年 / 33卷

基金：

中国国家自然科学基金;

关键词：

MicroRNA; Rolling circle amplification; Quantum dots; Anodic stripping voltammetric; HIGHLY SENSITIVE DETECTION; NUCLEIC-ACIDS; HYBRIDIZATION; SYSTEM; PROBES; ASSAY;

D O I：

10.1016/j.elecom.2013.04.030

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

MicroRNA-16 (miR-16) frequently deleted or downregulated in patients with B cell chronic lymphocytic leukemia and thus can be used as promising biomarkers in B-CLL diagnosis and prognosis. Herein, an ultrasensitive miR-16 detection was carried out by combining rolling circle amplification (RCA), quantum dots tagging and anodic stripping voltammetric detection (ASV). The method could specifically quantify miR-16 over a 6-decade dynamic range and reach an ultra-low detection limit of 032 aM. (c). 2013 Elsevier B.V. All rights reserved.

引用

页码：80 / 83

页数：4

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