Development of approaches to creation of experimental test system for evaluation of antigenic activity of synthetic oligosaccharide ligands related to fragments of the Streptococcus pneumoniae type 14 capsular polysaccharide

被引：13
作者：
Kurbatova, E. A. [1 ]
Vorobiov, D. S. [1 ]
Semenova, I. B. [1 ]
Sukhova, E. V. [2 ]
Yashunsky, D. V. [2 ,3 ]
Tsvetkov, Y. E. [2 ]
Nifantiev, N. E. [2 ]
机构：
[1] Russian Acad Med Sci, Mechnikov Res Inst Vaccines & Sera, Moscow 105064, Russia
[2] Russian Acad Sci, Lab Glycoconjugate Chem, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[3] Russian Acad Med Sci, Orekhovich Res Inst Biomed Chem, Moscow 119121, Russia
来源：
BIOCHEMISTRY-MOSCOW | 2013年 / 78卷 / 07期
基金：
俄罗斯基础研究基金会;
关键词：
Streptococcus pneumoniae type 14; capsular polysaccharide; synthetic oligosaccharide fragment; oligosaccharide-BSA conjugate; antigenic activity; RESISTANCE; CHEMISTRY; SEROTYPE; CHILDREN; VACCINE; DISEASE;
D O I：
10.1134/S0006297913070122
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A conjugate of a synthetic hexasaccharide fragment of the Streptococcus pneumoniae type 14 capsular polysaccharide with bovine serum albumin (BSA) has been prepared. The antigenic activity and specificity of this conjugate are comparable with those of natural antigens of S. pneumoniae type 14. The data suggest that the resulting synthetic conjugate can be used as a coating antigen in an experimental test system (based on enzyme immunoassay) for evaluating the antigenic activity and specificity of synthetic oligosaccharide ligands and for testing specimens of natural capsular polysaccharides and immune sera.
引用
收藏
页码：818 / 822
页数：5
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