Bacillus cereus biofilm formation on central venous catheters of hospitalised cardiac patients

被引:18
|
作者
Ikram, Samman [1 ,2 ,3 ]
Heikal, Adam [2 ,3 ]
Finke, Sarah [2 ,3 ]
Hofgaard, Antje [4 ]
Rehman, Yasir [1 ]
Sabri, Anjum Nasim [1 ]
Okstad, Ole Andreas [2 ,3 ]
机构
[1] Univ Punjab, Dept Microbiol & Mol Genet, Lahore, Pakistan
[2] Univ Oslo, Ctr Integrat Microbial Evolut, Dept Pharm, Oslo, Norway
[3] Univ Oslo, Sect Pharmacol & Pharmaceut Biosci, Dept Pharm, Oslo, Norway
[4] Univ Oslo, Dept Biosci, Oslo, Norway
关键词
Biofilm; central venous catheters; cardiac patients; Bacillus cereus group; whole genome sequencing; comparative genomics; GENOME SEQUENCE; NOSOCOMIAL BACTEREMIA; ANTHRACIS; THURINGIENSIS; ALIGNMENT; MYCOIDES; STRAINS; VERSION; MODEL; DNA;
D O I
10.1080/08927014.2019.1586889
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Formation of bacterial biofilms is a risk with many in situ medical devices. Biofilm-forming Bacillus species are associated with potentially life-threatening catheter-related blood stream infections in immunocompromised patients. Here, bacteria were isolated from biofilm-like structures within the lumen of central venous catheters (CVCs) from two patients admitted to cardiac hospital wards. Isolates belonged to the Bacillus cereus group, exhibited strong biofilm formation propensity, and mapped phylogenetically close to the B. cereus emetic cluster. Together, whole genome sequencing and quantitative PCR confirmed that the isolates constituted the same strain and possessed a range of genes important for and up-regulated during biofilm formation. Antimicrobial susceptibility testing demonstrated resistance to trimethoprim-sulphamethoxazole, clindamycin, penicillin and ampicillin. Inspection of the genome revealed several chromosomal -lactamase genes and a sulphonamide resistant variant of folP. This study clearly shows that B. cereus persisting in hospital ward environments may constitute a risk factor from repeated contamination of CVCs.
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页码:204 / 216
页数:13
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