Bacillus cereus biofilm formation on central venous catheters of hospitalised cardiac patients

被引:18
|
作者
Ikram, Samman [1 ,2 ,3 ]
Heikal, Adam [2 ,3 ]
Finke, Sarah [2 ,3 ]
Hofgaard, Antje [4 ]
Rehman, Yasir [1 ]
Sabri, Anjum Nasim [1 ]
Okstad, Ole Andreas [2 ,3 ]
机构
[1] Univ Punjab, Dept Microbiol & Mol Genet, Lahore, Pakistan
[2] Univ Oslo, Ctr Integrat Microbial Evolut, Dept Pharm, Oslo, Norway
[3] Univ Oslo, Sect Pharmacol & Pharmaceut Biosci, Dept Pharm, Oslo, Norway
[4] Univ Oslo, Dept Biosci, Oslo, Norway
关键词
Biofilm; central venous catheters; cardiac patients; Bacillus cereus group; whole genome sequencing; comparative genomics; GENOME SEQUENCE; NOSOCOMIAL BACTEREMIA; ANTHRACIS; THURINGIENSIS; ALIGNMENT; MYCOIDES; STRAINS; VERSION; MODEL; DNA;
D O I
10.1080/08927014.2019.1586889
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Formation of bacterial biofilms is a risk with many in situ medical devices. Biofilm-forming Bacillus species are associated with potentially life-threatening catheter-related blood stream infections in immunocompromised patients. Here, bacteria were isolated from biofilm-like structures within the lumen of central venous catheters (CVCs) from two patients admitted to cardiac hospital wards. Isolates belonged to the Bacillus cereus group, exhibited strong biofilm formation propensity, and mapped phylogenetically close to the B. cereus emetic cluster. Together, whole genome sequencing and quantitative PCR confirmed that the isolates constituted the same strain and possessed a range of genes important for and up-regulated during biofilm formation. Antimicrobial susceptibility testing demonstrated resistance to trimethoprim-sulphamethoxazole, clindamycin, penicillin and ampicillin. Inspection of the genome revealed several chromosomal -lactamase genes and a sulphonamide resistant variant of folP. This study clearly shows that B. cereus persisting in hospital ward environments may constitute a risk factor from repeated contamination of CVCs.
引用
收藏
页码:204 / 216
页数:13
相关论文
共 50 条
  • [31] Role of Bacillus cereus biofilm formation behavior in virulence and pathogenic characteristics
    Park, Eun-Ji
    Hussain, Mohammad Shakhawat
    Elahi, Fazle
    Hossain, Arzina
    Oh, Deog Hwan
    JOURNAL OF FOOD SAFETY, 2023, 43 (06)
  • [32] Alternative modes of biofilm formation by plant-associated Bacillus cereus
    Gao, Tantan
    Foulston, Lucy
    Chai, Yunrong
    Wang, Qi
    Losick, Richard
    MICROBIOLOGYOPEN, 2015, 4 (03): : 452 - 464
  • [33] Impact of manganese and heme on biofilm formation of Bacillus cereus food isolates
    Hussain, Mohammad Shakhawat
    Kwon, Minyeong
    Oh, Deog-Hwan
    PLOS ONE, 2018, 13 (07):
  • [34] Characterization of the codY gene and its influence on biofilm formation in Bacillus cereus
    Yi-Huang Hsueh
    Eileen B. Somers
    Amy C. Lee Wong
    Archives of Microbiology, 2008, 189 : 557 - 568
  • [35] CARDIAC-TAMPONADE FROM CENTRAL VENOUS CATHETERS
    DEFALQUE, RJ
    CAMPBELL, C
    ANESTHESIOLOGY, 1979, 50 (03) : 249 - 252
  • [36] Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus
    Joaquín Caro-Astorga
    Elrike Frenzel
    James R. Perkins
    Ana Álvarez-Mena
    Antonio de Vicente
    Juan A. G. Ranea
    Oscar P. Kuipers
    Diego Romero
    npj Biofilms and Microbiomes, 6
  • [37] Bacillus cereus sensu lato biofilm formation and its ecological importance
    Lin, Yicen
    Briandet, Romain
    Kovacs, Akos T.
    BIOFILM, 2022, 4
  • [38] CARDIAC-TAMPONADE FROM CENTRAL VENOUS CATHETERS
    EDWARDS, H
    KING, TC
    ARCHIVES OF SURGERY, 1982, 117 (07) : 965 - 967
  • [39] Central venous catheters and cardiac tamponade in preterm infants
    Marco Pezzati
    Luca Filippi
    Gianna Chiti
    Carlo Dani
    Sauro Rossi
    Giovanna Bertini
    Firmino F. Rubaltelli
    Intensive Care Medicine, 2004, 30 : 2253 - 2256
  • [40] Central venous catheters and cardiac tamponade in preterm infants
    Pezzati, M
    Filippi, L
    Chiti, G
    Dani, C
    Rossi, S
    Bertini, G
    Rubaltelli, FF
    INTENSIVE CARE MEDICINE, 2004, 30 (12) : 2253 - 2256