A benzimidazole-based conducting polymer and a PMMA-clay nanocomposite containing biosensor platform for glucose sensing

被引:16
作者
Emre, Fatma Bilge [1 ]
Kesik, Melis [2 ]
Kanik, Fulya Ekiz [3 ]
Akpinar, Hava Zekiye [4 ]
Aslan-Gurel, Evren [5 ]
Rossi, Rene M. [5 ]
Toppare, Levent [2 ,3 ,4 ,6 ]
机构
[1] Inonu Univ, Fac Educ, TR-44280 Malatya, Turkey
[2] Middle E Tech Univ, Dept Chem, TR-06800 Ankara, Turkey
[3] Middle E Tech Univ, Dept Biotechnol, TR-06800 Ankara, Turkey
[4] Middle E Tech Univ, Dept Polymer Sci & Technol, TR-06800 Ankara, Turkey
[5] EMPA Swiss Fed Labs Mat Sci & Technol, Lab Protect & Physiol, St Gallen, Switzerland
[6] Middle E Tech Univ, Ctr Solar Energy Res & Applicat GUNAM, TR-06800 Ankara, Turkey
关键词
Conducting polymer; Polymer clay nanocomposite; Laponite; Glucose biosensor; Immobilization platform; NYLON 6-CLAY HYBRID; ELECTROCHEMICAL POLYMERIZATION; BIOMOLECULE IMMOBILIZATION; MODIFIED ELECTRODES; PI-STACKING; LAPONITE; FILMS; POLYPYRROLE; EFFICIENCY; OXIDASE;
D O I
10.1016/j.synthmet.2015.06.015
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Development of materials composed of polymer-clay nanocomposites (PCN) and conducting polymers attracts great interest and preferred in various applications. Hereby, polymethylmethacrylate (PMMA) layered silicate nanocomposites were prepared by in-situ suspension polymerization by grafting PMMA with laponite using a suitable grafting agent. The properties of the as-synthesized PCN materials are characterized by differential scanning calorimetry (DSC), thermal gravimetry analysis (TGA) and gel permeation chromatography (GPC). A conducting polymer; poly(4-(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-y1)-7-(2,3-dihydrothieno[3,4-b][1,4]dioxin-7-y1)-2-benzyl-1H-benzo[d]imidazole) (poly(BIPE)) and a PMMA-clay nanocomposite with 2-(methacryloyloxy) ethyltrimethylammonium chloride (MTMA) modifier were examined as a platform for biomolecule deposition. Glucose oxidase (GOx, beta-D-glucose: oxygen-1-oxidoreductase, EC 1.1.3.4) was chosen as the model enzyme to prepare a scaffold for glucose sensing. Three different sensing strategies; PCN/GOx, poly(BIPE)/GOx and PCN/poly(BIPE)/GOx were analyzed and their biosensor performances were discussed. Surface morphology of the modified electrodes was characterized by scanning electron microscopy (SEM) technique. Electrochemical responses of the enzyme electrodes were monitored at -0.7 V vs. Ag reference electrode by monitoring oxygen consumption in the presence of glucose. After optimum conditions were determined, kinetic and analytical parameters; K-M(aPP), I-max, LOD and sensitivity were investigated for each sensing platform. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:102 / 109
页数:8
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