An ICT lighten ratiometric and NIR fluorogenic probe to visualize endogenous/exogenous hydrogen sulphide and imaging in mice

In recent years, due to the development of the field of fluorescent labeling and the importance of hydrogen sulfide (H2S) in biological systems, H2S fluorescence detection and bio-imaging have become popular. Especially, near-infrared or ratiometric fluorescence probe are booming because of their respective superiority. In this work, we integrated NIR and ratiometric (17-fold I-650/I-565) fluorescent emissions into probe molecular to detect H2S in pH 7.4 PBS buffer solution based on ICT lighten with high sensitivity (detection limit 1.08 nM) and fast response time (less than 120 s). In addition, the detection process can be monitored by naked-eye (from yellow to red). Thus the probe can image the concentration distribution of H2S in living cancer cells. NIR fluorescent probe has good tissue penetration, which was successfully applied in mice imaging.