Can a mixed damage interfere with DNA-protein cross-links repair?

被引：1

作者：

Marzano, C

Severin, E

Bordin, F

机构：

[1] Univ Padua, Dipartimento Sci Farmaceut, I-35131 Padua, Italy

[2] CNR, Ctr Studio Chim Farmaco & Prodotti Biol Attivi, Padua, Italy

[3] Carol Davila Univ Med & Pharm, Dept Genet, Bucharest, Romania

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2001年 / 5卷 / 02期

关键词：

furoquinolinones; DNA-protein cross-links; DNA fragmentation; chromosomal aberrations;

D O I：

10.1111/j.1582-4934.2001.tb00150.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Some photochemical and photobiological properties of 4,5',8-trimethylpsoralen (TMP) have been studied in comparison with 1,4,6,8-tetramethyl-2H-furo[2,3-h]quinolin-2 one (FQ) and 8-methoxypsoralen (8-MOP). The TMP and FQ can photobind to mammalian cell DNA in vivo, by UVA irradiation, forming DNA-protein cross-links (DPC), but only TMP shows a strong capacity of inducing interstrand cross-links (ISC). The mechanism of DPC formation was studied using the double irradiation method in Chinese hamster ovary (CHO) cells, and DPC were detected by alkaline elution. Both TMP and FQ induce covalent diadducts linking together DNA and proteins. Studying the formation of double strand breaks (DSB) in CHO cells we observed that TMP induced a low amount of DSB, similar to 8-MOP. TMP and 8-MOP induced chromosomal aberrations in CHO cells to the same extent, while FQ appeared to be more active. Our data suggest that the ISC induced by TMP could trap enzymes involved in DPC repair.

引用

页码：171 / 177

页数：7

共 15 条

[1] CONVERSION OF PSORALEN DNA MONOADDUCTS IN ESCHERICHIA-COLI TO INTER-STRAND DNA CROSS LINKS BY NEAR UV LIGHT (320-360 NM) - INABILITY OF ANGELICIN TO FORM CROSS LINKS, INVIVO [J].