Cloning and characterization of the groE locus from Actinobacillus pleuropneumoniae

被引：7
作者：
Vezina, G
Sirois, M
Clairoux, N
Boissinot, M
机构：
[1] UNIV QUEBEC, DEPT CHIM BIOL, TROIS RIVIERES, PQ G9A 5H7, CANADA
[2] UNIV LAVAL, Ste Foy, PQ G1K 7P4, CANADA
来源：
FEMS MICROBIOLOGY LETTERS | 1997年 / 147卷 / 01期
基金：
英国医学研究理事会;
关键词：
chaperone protein; heat shock; swine pathogen; genetic complementation; alcohol dehydrogenase (adhE); purine nucleoside phosphorylase (deoD); Actinobacillus pleuropneumoniae;
D O I：
10.1016/S0378-1097(96)00477-6
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A 4.4-kb DNA fragment was cloned from Actinobacillus pleuropneumoniae (strain 4074, serotype 1) by genetic complementation with Escherichia coli groES-groEL mutant strains, Sequence analysis of this fragment revealed a purine nucleoside phosphorylase (DeoD)-encoding gene homolog (deoD), heat-shock response-encoding genes for the small (groES) and large subunits (groEL) and a partial open reading frame encoding an alcohol dehydrogenase homolog (adhE), The predicted amino-acid sequence of groES and groEL genes showed extensive sequence identity (80-95%) with other Pasteurellaceae. The gene organization surrounding the groE locus was different from that of Haemophilus influenzae. When expressed in E. coli, groES-groEL genes were capable of complementing the growth of a lambda lytic phage, indicating a structural as well as functional conservation.
引用
收藏
页码：11 / 16
页数：6
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