Determination of polyunsatured fatty acid monoepoxides by high performance liquid chromatography-mass spectrometry

被引：15

作者：

Fer, Maude ^{[1
]}

Goulitquer, Sophie ^{[1
]}

Dreano, Yvonne ^{[1
]}

Berthou, Francois ^{[1
]}

Corcos, Laurent ^{[1
]}

Amet, Yolande ^{[1
]}

机构：

[1] Fac Med, Biochim Lab, F-29238 Brest 3, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1115卷 / 1-2期

关键词：

eicosatrienoic acid; arachidonic acid; eicosapentaenoic acid; docosahexaenoic acid; monoepoxides; regioisomers; ion-pair HPLC; LC-MS;

D O I：

10.1016/j.chroma.2006.02.060

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite the implication of polyunsaturated fatty acid monoepoxides in a large panel of biological effects, few methods allowing their separation in a single run are available. We describe here a simple method based on reversed-phase ion-pair high-performance liquid chromatography (RP-HPLC) and developed to successfully separate the various monoepoxides of eicosatrienoic, arachidonic, eicosapentaenoic and docosahexaenoic acids. These compounds were easily identified by liquid chromatography-mass spectrometry (LC-MS) with atmospheric pressure chemical ionisation owing to the volatility of counter-ion species. Compared to established methods, this new protocol proved its ability to totally resolve, in a single run, all of the different regioisomeric epoxides. In the long run, this method will demonstrate its efficacy to give insights into the cytochrome P450-dependent metabolism of polyunsatured fatty acids (PUFAs) and the generation of physiologically active epoxy-derivatives. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1 / 7

页数：7

共 26 条

[1] Eicosapentaenoic acid metabolism by cytochrome P450 enzymes of the CYP2C subfamily [J].