Chondroitin sulfate/dermatan sulfate from corb (Sciaena umbra) skin: Purification, structural analysis and anticoagulant effect

被引：35
作者：
Bougatef, Hajer [1 ]
Krichen, Fatma [1 ]
Capitani, Federica [2 ]
Ben Amor, Ikram [3 ]
Maccari, Francesca [2 ]
Mantovani, Veronica [2 ]
Galeotti, Fabio [2 ]
Volpi, Nicola [2 ]
Bougatef, Ali [1 ]
Sila, Assaad [1 ]
机构：
[1] Univ Sfax, Natl Sch Engn Sfax ENIS, Lab Improvement Plants & Valorizat Agroressources, Sfax 3038, Tunisia
[2] Univ Modena & Reggio Emilia, Dept Life Sci, Modena, Italy
[3] Reg Ctr Blood Transfus Sfax, El Ain Rd Km 0-5, Sfax 3003, Tunisia
来源：
CARBOHYDRATE POLYMERS | 2018年 / 196卷
关键词：
Fish skin; Glycosaminoglycans; Chondroitin sulfate; Dermatan sulfate; Chemical characterization; Anticoagulant effect; ACID CARBAZOLE REACTION; BLOOD-COAGULATION; FISH SKINS; IN-VITRO; GLYCOSAMINOGLYCANS; POLYSACCHARIDES; ELECTROPHORESIS; GREEN;
D O I：
10.1016/j.carbpol.2018.05.019
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In this study, chondroitin sulfate/dermatan sulfate was isolated and purified from the skin of corb (Sciaena umbra) (CSG) with a yield of 6.2%. Chemical and structural analysis showed that CSG consisted of high sulfate content 28.74% and an average molecular weight of 15.46 KDa. The separation of CSG by agarose-gel electrophoresis revealed the presence of DS and CS. Structural analysis of the purified CS/DS by means of SAX-HPLC after treatment with specific chondroitinases showed that this polymer was composed of nonsulfated disaccharide, monosulfated disaccharides and disulfated disaccharides in various percentages. The results also suggest that the percentage of CS and DS recovred in CSG were 24% and 76%, respectively. Anticoagulant activity in vitro was measured in plasma using classical anticoagulation tests: activated partial thromboplastin time (aPTT), thrombin time (TT) and prothrombine time (PT) tests. The findings thus indicated that the purified CS/DS exhibits a remarkably high anticoagulant effect.
引用
收藏
页码：272 / 278
页数：7
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