The acetate regulatory gene facB of Aspergillus nidulans encodes a Zn(II)2Cys6 transcriptional activator

被引:59
|
作者
Todd, RB
Murphy, RL
Martin, HM
Sharp, JA
Davis, MA
Katz, ME
Hynes, MJ
机构
[1] UNIV MELBOURNE, DEPT GENET, PARKVILLE, VIC 3052, AUSTRALIA
[2] UNIV BRITISH COLUMBIA, BIOTECHNOL LAB, VANCOUVER, BC V6T 1Z3, CANADA
[3] UNIV ADELAIDE, DEPT GENET, ADELAIDE, SA 5005, AUSTRALIA
[4] UNIV NEW ENGLAND, DEPT BIOCHEM, ARMIDALE, NSW 2351, AUSTRALIA
来源
MOLECULAR AND GENERAL GENETICS | 1997年 / 254卷 / 05期
基金
澳大利亚研究理事会;
关键词
acetate metabolism; facB; Zn(II)2CysG (C6 zinc) binuclear cluster; Aspergillus; gene regulation;
D O I
10.1007/s004380050444
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genetic studies have indicated that the facB gene of Aspergillus nidulans is a major regulatory gene involved in acetamide and acetate utilisation. Sequencing of the facB gene revealed that it encodes a protein that contains an N-terminal GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster for DNA binding, leucine zipper-like heptad repeat motifs and central and C-terminal acidic alpha-helical regions, consistent with a function as a DNA-binding transcriptional activator. The Zn(II)2CysG cluster shows strong similarity with those of the Saccharmyces cerevisiae carbon metabolism regulatory proteins CAT8 and SIP4. A significant level of similarity with CAT8 is found throughout the length of the protein, suggesting at least partial functional homology. The facB genes of Aspergillus oryzae and Aspergillus niger were also sequenced and found to be highly conserved. Deletion of the facB gene confirmed that it is required for growth on acetate as a sole carbon source. Functional dissection using deletion and fusion constructs and in vitro mutagenesis indicated that the Zn(II)2Cys6 cluster and the C-terminal end of the protein are required for function.
引用
收藏
页码:495 / 504
页数:10
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