Development of an immunochromatographic assay for the rapid detection of bromoxynil in water

被引:50
|
作者
Zhu, Jiang [1 ]
Chen, Wenchao [2 ]
Lu, Yitong [1 ]
Cheng, Guohua [1 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China
[2] Univ Nottingham, Sch Biosci, Loughborough LE12 5RD, Leics, England
基金
中国国家自然科学基金;
关键词
Bromoxynil; Gold immunochromatographic assay (GICA); Colloidal gold; One-step strip test; Pesticide residue;
D O I
10.1016/j.envpol.2007.12.020
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A rapid immunochromatographic one-step strip test was developed to specifically determine bromoxynil in surface and drinking water by competitive inhibition with the nano colloidal gold-conjugated monoclonal antibody (mAb). Bromoxynil standard samples of 0.01-10 mg L-1 in water were tested by this method and the visual limit was 0.06 mg L-1. The assay only required 5 min and one-step by dispensing a drop of sample solution onto a strip. Parallel analysis of water samples with bromoxynil showed comparable results from one-step strip test and ELISA. Therefore, the one-step strip test is very useful as a screening method for qualitative detection of bromoxynil in water. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:136 / 142
页数:7
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