Pluripotent Stem Cell-Derived Somatic Stem Cells as Tool to Study the Role of MicroRNAs in Early Human Neural Development

被引:0
|
作者
Roese-Koerner, B.
Stappert, L.
Koch, P.
Bruestle, O. [1 ]
Borghese, L.
机构
[1] Univ Bonn, LIFE & BRAIN Ctr, Inst Reconstruct Neurobiol, D-53127 Bonn, Germany
关键词
Development; microRNA; miR-124; miR-125b; miR-9/9*; neural stem cell; pluripotent stem cell; NEURONAL DIFFERENTIATION; HUMAN ES; EARLY NEUROGENESIS; HUMAN FIBROBLASTS; REGULATORY RNAS; NONCODING RNAS; IPS CELLS; IN-VITRO; EXPRESSION; MIR-124;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The in vitro differentiation of human pluripotent stem cells represents a convenient approach to generate large numbers of neural cells for basic and translational research. We recently described the derivation of homogeneous populations of long-term self-renewing neuroepithelial-like stem cells from human pluripotent stem cells (lt-NES (R) cells). These cells constitute a suitable source of neural stem cells for in vitro modelling of early human neural development. Recent evidence demonstrates that microRNAs are important regulators of stem cells and nervous system development. Studies in several model organisms suggest that microRNAs contribute to different stages of neurogenesis - from progenitor self-renewal to survival and function of differentiated neurons. However, the understanding of the impact of microRNA-based regulation in human neural development is still at its dawn. Here, we give an overview on the current state of microRNA biology in stem cells and neural development and examine the role of the neural-associated miR-124, miR-125b and miR-9/9* in human lt-NES (R) cells. We show that overexpression of miR-124, as well as overexpression of miR-125b, impair lt-NES (R) cell self-renewal and induce differentiation into neurons. Overexpression of the miR-9/9* locus also impairs self-renewal of lt-NES (R) cells and supports their commitment to neuronal differentiation. A detailed examination revealed that overexpression of miR-9 promotes differentiation, while overexpression of miR-9* affects both proliferation and differentiation of lt-NES (R) cells. This work provides insights into the regulation of early human neuroepithelial cells by microRNAs and highlights the potential of controlling differentiation of human stem cells by modulating the expression of selected microRNAs.
引用
收藏
页码:707 / 722
页数:16
相关论文
共 50 条
  • [1] Effect of Cell Spreading on Rosette Formation by Human Pluripotent Stem Cell-Derived Neural Progenitor Cells
    Townshend, Ryan F.
    Shao, Yue
    Wang, Sicong
    Cortez, Chari L.
    Esfahani, Sajedeh Nasr
    Spence, Jason R.
    O'Shea, K. Sue
    Fu, Jianping
    Gumucio, Deborah L.
    Taniguchi, Kenichiro
    FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2020, 8
  • [2] Pig Induced Pluripotent Stem Cell-Derived Neural Rosettes Developmentally Mimic Human Pluripotent Stem Cell Neural Differentiation
    Gallegos-Cardenas, Amalia
    Webb, Robin
    Jordan, Erin
    West, Rachel
    West, Franklin D.
    Yang, Jeong-Yeh
    Wang, Kai
    Stice, Steven L.
    STEM CELLS AND DEVELOPMENT, 2015, 24 (16) : 1901 - 1911
  • [3] In vitro segregation and isolation of human pluripotent stem cell-derived neural crest cells
    Muenst, Sabine
    Koch, Philipp
    Kesavan, Jaideep
    Alexander-Mays, Michael
    Muenst, Bernhard
    Blaess, Sandra
    Bruestle, Oliver
    METHODS, 2018, 133 : 65 - 80
  • [4] Integrated Platform for Production and Purification of Human Pluripotent Stem Cell-Derived Neural Precursors
    Rodrigues, Goncalo M. C.
    Matos, Andreia F. S.
    Fernandes, Tiago G.
    Rodrigues, Carlos A. V.
    Peitz, Michael
    Haupt, Simone
    Diogo, Maria Margarida
    Bruestle, Oliver
    Cabral, Joaquim M. S.
    STEM CELL REVIEWS AND REPORTS, 2014, 10 (02) : 151 - 161
  • [5] Human Induced Pluripotent Stem Cell-Derived Models to Investigate Human Cytomegalovirus Infection in Neural Cells
    D'Aiuto, Leonardo
    Di Maio, Roberto
    Heath, Brianna
    Raimondi, Giorgio
    Milosevic, Jadranka
    Watson, Annie M.
    Bamne, Mikhil
    Parks, W. Tony
    Yang, Lei
    Lin, Bo
    Miki, Toshio
    Mich-Basso, Jocelyn Danielle
    Arav-Boger, Ravit
    Sibille, Etienne
    Sabunciyan, Sarven
    Yolken, Robert
    Nimgaonkar, Vishwajit
    PLOS ONE, 2012, 7 (11):
  • [6] Induced pluripotent stem cell-derived neural stem cells: new hope for stroke?
    Liu, Jia
    STEM CELL RESEARCH & THERAPY, 2013, 4
  • [7] Induced pluripotent stem cell-derived neural stem cells: new hope for stroke?
    Jia Liu
    Stem Cell Research & Therapy, 4
  • [8] Human pluripotent stem cell-derived neural constructs for predicting neural toxicity
    Schwartz, Michael P.
    Hou, Zhonggang
    Propson, Nicholas E.
    Zhang, Jue
    Engstrom, Collin J.
    Costa, Vitor Santos
    Jiang, Peng
    Nguyen, Bao Kim
    Bolin, Jennifer M.
    Daly, William
    Wang, Yu
    Stewart, Ron
    Page, C. David
    Murphy, William L.
    Thomson, James A.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (40) : 12516 - 12521
  • [9] The Role of microRNAs in Neural Stem Cells and Neurogenesis
    Ji, Fen
    Lv, Xiaohui
    Jiao, Jianwei
    JOURNAL OF GENETICS AND GENOMICS, 2013, 40 (02) : 61 - 66
  • [10] Hydrogel-Based Bioprocess for Scalable Manufacturing of Human Pluripotent Stem Cell-Derived Neural Stem Cells
    Ling, Haishuang
    Du, Qian
    Li, Qian
    Wang, Ou
    Wang, Zhanqi
    Liu, Kan
    Elowsky, Christian
    Zhang, Chi
    Lei, Yuguo
    ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (35) : 29238 - 29250