Loop-mediated isothermal amplification-microfluidic chip for the detection of Trichophyton infection

被引：2

作者：

Jiang, Weiwei ^{[1
]}

Hu, Dongying ^{[1
]}

Xu, Yanyan ^{[2
]}

Chen, Yang ^{[1
]}

Zhu, Xiaoyang ^{[1
]}

Han, Zhao ^{[2
]}

Ye, Xin ^{[3
]}

Li, Xiaojing ^{[2
]}

机构：

[1] 72nd Grp Army Hosp PLA, Dept Dermatol, Huzhou, Zhejiang, Peoples R China

[2] Hebei Univ Engn, Affiliated Hosp, Handan, Hebei, Peoples R China

[3] Xi An Jiao Tong Univ, Dept Lab Med, Affiliated Hosp 1, Xian, Shaanxi, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2022年 / 13卷

关键词：

Trichophyton; LAMP; microfluidics; early diagnosis; chip; TINEA-CAPITIS; IDENTIFICATION; ONYCHOMYCOSIS; TERBINAFINE; GUIDELINES; MANAGEMENT; DIAGNOSIS; SKIN;

D O I：

10.3389/fmicb.2022.1031388

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Trichophyton is the most pathogenic type of fungal skin infection. It often invades and grows in a keratin-rich matrix, and lesions include human skin, hair, and fingernails (toenails). We designed LAMP primers for Trichophyton and developed a LAMP-Microfluidic chip detection system for Trichophyton. This system detects six common species of Trichophyton in the genus Trichophyton, including Trichophyton rubrum, Trichophyton mentagrophyte, Trichophyton violaceum, Trichophyton tonsurans, Trichophyton verrucosum, and Trichophyton schoenleinii. The specificity reached 100%, and the sensitivity could reach about 1 x 10(2) copies/mu l. The entire detection process can be completed within 60 min and does not cross-react with other dermatophytes. The established LAMP-Microfluidic chip detection system has the advantages of simple operation, high specificity, and high sensitivity, and has the potential for clinical application.

引用

页数：9