The genus Pterospermum Schreb. (Malvaceae) with 54 species worldwide and it is represented by 11 taxa in India. Pterospermum reticulatum Wight and Arn. is listed as vulnerable B1+2c species by IUCN. The mitotic chromosome number 2n=38 and karyomorphology of P. reticulatum are reported for the first time. The karyotype formula is 18 m+1sm and belongs to 2B karyotype of the Stebbins category.