Identification and intracellular localization of carbonic anhydrase I in gills, heart, muscle, and intestine of rainbow trout, Oncorhynchus mykiss

被引:2
作者
Kho, Kang Hee [1 ]
Kim, Jung Woo [2 ]
Kim, Soo Cheol [3 ]
Choi, Myeong Rak [3 ]
Han, Kyeong Ho [1 ]
Lee, Won Kyo [1 ]
Choi, Kap Seong [4 ]
机构
[1] Chonnam Natl Univ, Dept Aquat Biol, Yeosu 550749, South Korea
[2] Seonam Univ, Coll Med, Dept Anat, Namwon 590711, South Korea
[3] Chonnam Natl Univ, Dept Biomed & Elect Engn, Yeosu 550749, South Korea
[4] Sunchon Natl Univ, Dept Food Sci & Technol, Sunchon 540950, South Korea
来源
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY | 2015年 / 58卷 / 05期
关键词
Carbonic anhydrase I; Gill; Heart; Intestine; Muscle; Rainbow trout; ACID-BASE REGULATION; GAS-EXCHANGE; EXPRESSION; INHIBITION; TRANSPORT;
D O I
10.1007/s13765-015-0098-7
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Carbonic anhydrases (CAs) play a key role in physiological functions such as pH homeostasis, calcification, photosynthesis, and ionic regulations. Among CA isozymes, CA I is a specific marker for the cytoplasm or apical cell membranes of colonic epithelial cells, and it is related to enterocyte proliferation in mammals. The major aim of the present study was to investigate the regional, cellular, and subcellular localization of CA I in a fish, rainbow trout, Oncorhynchus mykiss. The CA I was identified in the gills, heart, and intestine of the rainbow trout. CA I protein detected in the tissues of the rainbow trout was similar to a CA III with a molecular weight of 29 kDa. Immunohistochemical results demonstrated that CA I was localized in the various cells of gills, heart, muscle, and intestine of the rainbow trout.
引用
收藏
页码:729 / 733
页数:5
相关论文
共 50 条
  • [1] Identification and intracellular localization of carbonic anhydrase I in gills, heart, muscle, and intestine of rainbow trout, Oncorhynchus mykiss
    Kang Hee Kho
    Jung Woo Kim
    Soo Cheol Kim
    Myeong Rak Choi
    Kyeong Ho Han
    Won Kyo Lee
    Kap Seong Choi
    Journal of the Korean Society for Applied Biological Chemistry, 2015, 58 : 729 - 733
  • [2] Kinetic properties of carbonic anhydrase purified from gills of rainbow trout (Oncorhynchus mykiss)
    Hisar, O.
    Beydemir, S.
    Bulbul, M.
    Yanik, T.
    JOURNAL OF APPLIED ANIMAL RESEARCH, 2006, 30 (02) : 185 - 188
  • [3] Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus mykiss
    Rahim, S. M.
    Mazlan, A. G.
    Simon, K. D.
    Delaunoy, J. P.
    Laurent, P.
    JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B, 2014, 15 (02): : 194 - 200
  • [4] Inhibition effect of cadmium on carbonic anhydrase in rainbow trout (Oncorhynchus mykiss)
    Bektas, Serdar
    Hisar, Oleay
    Hisar, Suekriye Aras
    Yanik, Telat
    FRESENIUS ENVIRONMENTAL BULLETIN, 2008, 17 (7A): : 793 - 796
  • [5] In vitro and in vivo effects of some pesticides on carbonic anhydrase enzyme from rainbow trout (Oncorhynchus mykiss) gills
    Ceyhun, Saltuk Bugrahan
    Senturk, Murat
    Erdogan, Orhan
    Kufrevioglu, Omer Irfan
    PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2010, 97 (03) : 177 - 181
  • [6] Intracellular carbonic anhydrase contributes to the red blood cell adrenergic response in rainbow trout Oncorhynchus mykiss
    Carrie, D. W.
    Gilmour, K. M.
    RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY, 2012, 184 (01) : 60 - 64
  • [7] Localization and diurnal variations of carbonic anhydrase rnRNA expression in the inner ear of the rainbow trout Oncorhynchus mykiss
    Tohse, Hidekazu
    Murayama, Emi
    Ohira, Tsuyoshi
    Takagi, Yasuaki
    Nagasawa, Hiromichi
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2006, 145 (3-4): : 257 - 264
  • [8] The impact of heavy metals on the activity of carbonic anhydrase from rainbow trout (Oncorhynchus mykiss) kidney
    Soyut, Hakan
    Beydemir, Sukru
    TOXICOLOGY AND INDUSTRIAL HEALTH, 2012, 28 (04) : 296 - 305
  • [9] Roles of cortisol and carbonic anhydrase in acid-base compensation in rainbow trout, Oncorhynchus mykiss
    Gilmour, K. M.
    Collier, C. L.
    Dey, C. J.
    Perry, S. F.
    JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY, 2011, 181 (04): : 501 - 515
  • [10] The role of renal carbonic anhydrase in acid-base regulation in rainbow trout, Oncorhynchus mykiss
    Gilmour, K. M.
    Georgalis, T.
    Swenson, E. R.
    Perry, S. F.
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2005, 141 (03): : S181 - S181