Identification and intracellular localization of carbonic anhydrase I in gills, heart, muscle, and intestine of rainbow trout, Oncorhynchus mykiss

被引：3

作者：

Kho, Kang Hee ^{[1
]}

Kim, Jung Woo ^{[2
]}

Kim, Soo Cheol ^{[3
]}

Choi, Myeong Rak ^{[3
]}

Han, Kyeong Ho ^{[1
]}

Lee, Won Kyo ^{[1
]}

Choi, Kap Seong ^{[4
]}

机构：

[1] Chonnam Natl Univ, Dept Aquat Biol, Yeosu 550749, South Korea

[2] Seonam Univ, Coll Med, Dept Anat, Namwon 590711, South Korea

[3] Chonnam Natl Univ, Dept Biomed & Elect Engn, Yeosu 550749, South Korea

[4] Sunchon Natl Univ, Dept Food Sci & Technol, Sunchon 540950, South Korea

来源：

JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY | 2015年 / 58卷 / 05期

关键词：

Carbonic anhydrase I; Gill; Heart; Intestine; Muscle; Rainbow trout; ACID-BASE REGULATION; GAS-EXCHANGE; EXPRESSION; INHIBITION; TRANSPORT;

D O I：

10.1007/s13765-015-0098-7

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Carbonic anhydrases (CAs) play a key role in physiological functions such as pH homeostasis, calcification, photosynthesis, and ionic regulations. Among CA isozymes, CA I is a specific marker for the cytoplasm or apical cell membranes of colonic epithelial cells, and it is related to enterocyte proliferation in mammals. The major aim of the present study was to investigate the regional, cellular, and subcellular localization of CA I in a fish, rainbow trout, Oncorhynchus mykiss. The CA I was identified in the gills, heart, and intestine of the rainbow trout. CA I protein detected in the tissues of the rainbow trout was similar to a CA III with a molecular weight of 29 kDa. Immunohistochemical results demonstrated that CA I was localized in the various cells of gills, heart, muscle, and intestine of the rainbow trout.

引用

页码：729 / 733

页数：5

共 23 条

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