Evaluation and implementation of a membrane filter method for Cronobacter detection in drinking water

被引：21

作者：

Liu, Hui ^{[1
]}

Yang, Yuelian ^{[1
]}

Cui, Jinghua ^{[2
]}

Liu, Lanzheng ^{[1
]}

Liu, Huiyuan ^{[1
]}

Hu, Guangchun ^{[1
]}

Shi, Yuwen ^{[1
]}

Li, Jian ^{[1
]}

机构：

[1] Jinan Municipal Ctr Decease Control & Prevent, Jinan 250021, Peoples R China

[2] Chinese Ctr Decease Control & Prevent, Beijing, Peoples R China

来源：

FEMS MICROBIOLOGY LETTERS | 2013年 / 344卷 / 01期

关键词：

membrane filter method; Cronobacter; drinking water; pulsed field gel electrophoresis; fusA; POWDERED INFANT FORMULA; ENTEROBACTER-SAKAZAKII; SP-NOV; COLIFORM; GENUS; FOOD;

D O I：

10.1111/1574-6968.12155

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A membrane filter (MF) method was evaluated for its suitability for qualitative and quantitative analyses of Cronobacter spp. in drinking water by pure strains of Cronobacter and non-Cronobacter, and samples spiked with chlorinated Cronobacter sakazakii ATCC 29544. The applicability was verified by the tests: for pure strains, the sensitivity and the specificity were both 100%; for spiked samples, the MF method recovered 82.8 +/- 10.4% chlorinated ATCC 29544 cells. The MF method was also applied to screen Cronobacter spp. in drinking water samples from municipal water supplies on premises (MWSP) and small community water supplies on premises (SCWSP). The isolation rate of Cronobacter spp. from SCWSP samples was 31/114, which was significantly higher than that from MWSP samples which was 1/131. Besides, the study confirmed the possibility of using total coliform as an indicator of contamination level of Cronobacter spp. in drinking water, and the acquired correct positive rate was 96%.

引用

页码：60 / 68

页数：9

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