Characterization of natural frequencies from nanoscale tissue oscillations using dynamic optical coherence elastography

We demonstrate the use of OCT-based elastography for soft-tissue characterization using natural frequency oscillations. Sub-micrometer to sub-manometer oscillations were induced in tissue phantoms and human cornea in vivo by perpendicular air-pulse stimulation and observed by common-path OCT imaging (sensitivity: 0.24 nm). Natural frequency and damping ratio were acquired in temporal and frequency domains using a single degree of freedom method. The dominant natural frequency was constant for different stimulation pressures (4-32 Pa) and measured distances (0.3-5.3 mm), and decreased as the sample thickness increased. The dominant natural frequencies of 0.75-2% agar phantoms were 127-774 Hz (mean coefficient of variation [CV]: 0.9%), and correlated with the square root of Young's moduli (16.5-117.8 kPa, mean CV: 5.8%). These preliminary studies show repeatable in vivo corneal natural frequency measurements (259 Hz, CV: 1.9%). This novel OCE approach can distinguish tissues and materials with different mechanical properties using the small-amplitude tissue oscillation features, and is suitable for characterizing delicate tissues in vivo such as the eye. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement