Response Surface Methodology Optimization and Kinetics of Diesel Degradation by a Cold-Adapted Antarctic Bacterium,Arthrobactersp. Strain AQ5-05

被引：34

作者：

Abdulrasheed, Mansur ^{[1
,2
]}

Zulkharnain, Azham ^{[3
]}

Zakaria, Nur Nadhirah ^{[1
]}

Roslee, Ahmad Fareez Ahmad ^{[1
]}

Khalil, Khalilah Abdul ^{[4
]}

Napis, Suhaimi ^{[5
]}

Convey, Peter ^{[6
]}

Gomez-Fuentes, Claudio ^{[7
]}

Ahmad, Siti Aqlima ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Biochem, Serdang 43400, Selangor, Malaysia

[2] Gombe State Univ, Fac Sci, Dept Microbiol, PMB 127, Gombe, Nigeria

[3] Shibaura Inst Technol, Dept Biosci & Engn, Coll Syst Engn & Sci, Minuma Ku, 307 Fukasaku, Saitama 3378570, Japan

[4] Univ Teknol MARA, Fac Appl Sci, Sch Biol, Shah Alam 40450, Selangor, Malaysia

[5] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Cell & Mol Biol, Serdang 43400, Selangor, Malaysia

[6] British Antarctic Survey, NERC, Madingley Rd, Cambridge CB3 0ET, England

[7] Univ Magallanes, Dept Chem Engn, Avda Bulnes, Punta Arenas 01855, Region De Magal, Chile

来源：

SUSTAINABILITY | 2020年 / 12卷 / 17期

基金：

英国自然环境研究理事会;

关键词：

Antarctica; Arthrobacter; diesel bioremediation; growth kinetics; response surface methodology; DEGRADING BACTERIA; PHENOL DEGRADATION; CHICKEN FEATHER; MONOD KINETICS; GROWTH; BIODEGRADATION; OIL; DESIGN; SOIL; BIOREMEDIATION;

D O I：

10.3390/su12176966

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Petroleum hydrocarbons, notably diesel oil, are the main energy source for running amenities in the Antarctic region and are the major cause of pollution in this area. Diesel oil spills are one of the major challenges facing management of the Antarctic environment. Bioremediation using bacteria can be an effective and eco-friendly approach for their remediation. However, since the introduction of non-native organisms, including microorganisms, into the Antarctic or between the distinct biogeographical regions within the continent is not permitted under the Antarctic Treaty, it is crucial to discover native oil-degrading, psychrotolerant microorganisms that can be used in diesel bioremediation. The primary aim of the current study is to optimize the conditions for growth and diesel degradation activity of an Antarctic local bacterium,Arthrobactersp. strain AQ5-05, using the Plackett-Burman approach and response surface method (RSM) via a central composite design (CCD) approach. Based on this approach, temperature, pH, and salinity were calculated to be optimum at 16.30 degrees C, pH 7.67 and 1.12% (w/v), respectively. A second order polynomial regression model very accurately represented the experimental figures' interpretation. These optimized environmental conditions increased diesel degradation from 34.5% (at 10 degrees C, pH 7.00 and 1.00% (w/v) salinity) to 56.4%. Further investigation of the kinetics of diesel reduction by strain AQ5-05 revealed that the Teissier model had the lowest RMSE and AICC values. The calculated values for the Teissier constants of maximal growth rate, half-saturation rate constant for the maximal growth, and half inhibition constants (mu(max), Ks, and Ki), were 0.999 h(-1), 1.971% (v/v) and 1.764% (v/v), respectively. The data obtained therefore confirmed the potential application of this cold-tolerant strain in the bioremediation of diesel-contaminated Antarctic soils at low temperature.

引用

页数：14

共 58 条

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