How paired PSII-LHCII supercomplexes mediate the stacking of plant thylakoid membranes unveiled by structural mass-spectrometry

被引:63
作者
Albanese, Pascal [1 ]
Tamara, Sem [2 ,3 ,4 ]
Saracco, Guido [1 ]
Scheltema, Richard A. [2 ,3 ,4 ]
Pagliano, Cristina [1 ]
机构
[1] Politecn Torino, BioSolar Lab, Appl Sci & Technol Dept, Environm Pk,Via Livorno 60, I-10144 Turin, Italy
[2] Univ Utrecht, Biomol Mass Spectrometry & Prote, Bijvoet Ctr Biomol Res, Padualaan 8, NL-3584 CH Utrecht, Netherlands
[3] Univ Utrecht, Utrecht Inst Pharmaceut Sci, Padualaan 8, NL-3584 CH Utrecht, Netherlands
[4] Netherlands Prote Ctr, Padualaan 8, NL-3584 CH Utrecht, Netherlands
基金
荷兰研究理事会; 欧盟地平线“2020”;
关键词
LIGHT-HARVESTING COMPLEX; CHEMICAL CROSS-LINKING; PHOTOSYSTEM-II; CRYSTAL-STRUCTURE; PROTEINS; VISUALIZATION; PHOSPHORYLATION; ARCHITECTURE; EVOLUTION; SPINACH;
D O I
10.1038/s41467-020-15184-1
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Grana are a characteristic feature of higher plants' thylakoid membranes, consisting of stacks of appressed membranes enriched in Photosystem II (PSII) and associated light-harvesting complex II (LHCII) proteins, together forming the PSII-LHCII supercomplex. Grana stacks undergo light-dependent structural changes, mainly by reorganizing the supramolecular structure of PSII-LHCII supercomplexes. LHCII is vital for grana formation, in which also PSII-LHCII supercomplexes are involved. By combining top-down and crosslinking mass spectrometry we uncover the spatial organization of paired PSII-LHCII supercomplexes within thylakoid membranes. The resulting model highlights a basic molecular mechanism whereby plants maintain grana stacking at changing light conditions. This mechanism relies on interactions between stroma-exposed N-terminal loops of LHCII trimers and Lhcb4 subunits facing each other in adjacent membranes. The combination of light-dependent LHCII N-terminal trimming and extensive N-terminal a-acetylation likely affects interactions between pairs of PSII-LHCII supercomplexes across the stromal gap, ultimately mediating membrane folding in grana stacks.
引用
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页数:14
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