Development of reagents to study the turkey's immune response: Cloning and characterisation of two turkey cytokines, interleukin (IL)-10 and IL-13

被引：7

作者：

Powell, Fiona ^{[1
]}

Rothwell, Lisa ^{[1
]}

Clarkson, Michael ^{[2
]}

Kaiser, Pete ^{[1
]}

机构：

[1] Inst Anim Hlth, Compton RG20 7NN, Berks, England

[2] Univ Liverpool, Leahurst CH64 7TE, Neston, England

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2012年 / 147卷 / 1-2期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Avian; Cytokine; Bioactivity; Cross-reactivity; CHICKEN INTERFERON-GAMMA; HIGH SEQUENCE IDENTITY; MONOCLONAL-ANTIBODIES; FUNCTIONAL HOMOLOGY; DISEASE VIRUS; INFECTION; GENOME; GENE; IDENTIFICATION; PROTEINS;

D O I：

10.1016/j.vetimm.2012.03.013

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The cDNAs of two turkey cytokines, interleukin (IL)-10 and IL-13, were cloned using oligonucleotide primers designed from their chicken orthologues. The coding regions of the chicken and turkey genes are highly conserved, with IL-10 and IL-13 exhibiting 94.1% and 90% nucleotide and 92% and 79.9% amino acid identity respectively. Both showed consistent mRNA expression in turkey lymphoid and gut tissues. Expression in non-lymphoid tissues was more variable but generally highest in the skin and trachea. Recombinant turkey IL-10 was expressed and bioactivity demonstrated by inhibition of IFN-gamma synthesis from activated splenocytes. Chicken and turkey IL-10 cross-reacted in functional assays. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：97 / 103

页数：7

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