Evaluation of an Inexpensive Growth Medium for Direct Detection of Escherichia coli in Temperate and Sub-Tropical Waters

被引：15

作者：

Bain, Robert E. S. ^{[1
]}

Woodall, Claire ^{[2
]}

Elliott, John ^{[3
]}

Arnold, Benjamin F. ^{[4
]}

Tung, Rosalind ^{[5
]}

Morley, Robert ^{[6
]}

du Preez, Martella ^{[7
]}

Bartram, Jamie K. ^{[8
]}

Davis, Anthony P. ^{[2
]}

Gundry, Stephen W. ^{[9
]}

Pedley, Stephen ^{[3
]}

机构：

[1] UNICEF, New York, NY 10038 USA

[2] Univ Bristol, Sch Chem, Bristol, Avon, England

[3] Univ Surrey, Dept Civil & Environm Engn, Guildford GU2 5XH, Surrey, England

[4] Univ Calif Berkeley, Sch Publ Hlth, Div Epidemiol, Berkeley, CA 94720 USA

[5] CPI Int, Santa Rosa, CA USA

[6] Publ Hlth England, Bristol, Avon, England

[7] CSIR, ZA-0001 Pretoria, South Africa

[8] Univ N Carolina, Water Inst, Chapel Hill, NC USA

[9] Brightwater Diagnost Ltd, Bath, Avon, England

来源：

PLOS ONE | 2015年 / 10卷 / 10期

基金：

比尔及梅琳达.盖茨基金会;

关键词：

BETA-D-GLUCURONIDASE; DRINKING-WATER; TOTAL COLIFORMS; FRESH-WATER; H2S TEST; ENUMERATION; TESTS; COLILERT-18; COUNTRIES; BACTERIA;

D O I：

10.1371/journal.pone.0140997

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cost and complexity of traditional methods for the detection of faecal indicator bacteria, including E. coli, hinder widespread monitoring of drinking water quality, especially in low-income countries and outside controlled laboratory settings. In these settings the problem is exacerbated by the lack of inexpensive media for the detection of E. coli in drinking water. We developed a new low-cost growth medium, aquatest (AT), and validated its use for the direct detection of E. coli in temperate and sub-tropical drinking waters using IDEXX Quanti-Tray (R). AT was compared with IDEXX Colilert-18 (R) and either EC-MUG or MLSB for detecting low levels of E. coli from water samples from temperate (n = 140; Bristol, UK) and subtropical regions (n = 50, Pretoria/Tshwane, South Africa). Confirmatory testing (n = 418 and 588, respectively) and the comparison of quantitative results were used to assess performance. Sensitivity of AT was higher than Colilert-18 (R) for water samples in the UK [98.0% vs. 86.9%; p<0.0001] and South Africa [99.5% vs. 93.2%; p = 0.0030]. There was no significant difference in specificity, which was high for both media (>95% in both settings). Quantitative results were comparable and within expected limits. AT is reliable and accurate for the detection of E. coli in temperate and subtropical drinking water. The composition of the new medium is reported herein and can be used freely.

引用

页数：13