Crystallization and preliminary X-ray crystallographic analyses of EcoO109I and its complex with DNA

被引：4

作者：

Imasaki, T

Hashimoto, H

Shimizu, T

Kato, M

Tsuda, J

Kita, K

Sato, M

机构：

[1] Yokohama City Univ, Grad Sch Integrated Sci, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[2] Tottori Univ, Dept Biotechnol, Tottori 6808552, Japan

[3] Kyoto Univ, Grad Sch Agr, Kyoto 6110011, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2004年 / 60卷

关键词：

D O I：

10.1107/S0907444904009412

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

EcoO109I is a type II restriction endonuclease that recognizes seven base pairs of the degenerate and discontinuous sequence RGGNCCY. The enzyme and its complex with DNA were successfully crystallized by the hanging-drop vapour-diffusion method using polyethylene glycols as precipitants. The crystal of EcoO109I belongs to space group I4, with unit-cell parameters a=b=175.5, c=44.6 Angstrom, and that of the DNA complex belongs to space group P2(1)2(1)2(1), with unit-cell parameters a=49.1, b=71.8, c=203.2 Angstrom. Full sets of X-ray diffraction data from the enzyme and its complex with DNA were collected to 2.4 and 1.9 Angstrom resolution, respectively.

引用

页码：1165 / 1166

页数：2

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