An assay for environmental nitrate that is based on its reduction to nitrite by nitrite reductase deficient Neurospora mycelia

被引：1
|
作者：
Io, R [1 ]
Pullen, J [1 ]
Zaimi, O [1 ]
Sorger, GJ [1 ]
机构：
[1] MCMASTER UNIV,DEPT BIOL,HAMILTON,ON L8S 4K1,CANADA
来源：
ENVIRONMENTAL TECHNOLOGY | 1996年 / 17卷 / 06期
关键词：
nitrate; measurement; neurospora; mutant; water;
D O I：
10.1080/09593331708616432
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A simple, sensitive, reliable and inexpensive assay for environmental nitrate in solution, based on its reduction to nitrite in vivo, by nitrite reductase deficient Neurospora mycelia, is described. Water samples are amended to contain ingredients necessary for the growth of Neurospora except nitrogen, which is provided by the endogenous nitrate. Mycelia are incubated with this amended water sample and allowed to convert the endogenous nitrate to nitrite. The nitrite is then measured colourimetrically.
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页码：661 / 666
页数：6
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