Integrated and automated high-throughput purification of libraries on microscale

被引：9

作者：

Ginsburg-Moraff, Carol ^{[2
]}

Grob, Jonathan ^{[2
,4
]}

Chin, Karl ^{[2
]}

Eastman, Grant ^{[2
]}

Wildhaber, Sandra ^{[1
]}

Bayliss, Mark ^{[3
]}

Mues, Heinrich M. ^{[1
]}

Palmieri, Marco ^{[1
]}

Poirier, Jennifer

Reck, Marcel ^{[1
]}

Luneau, Alexandre ^{[1
]}

Rodde, Stephane ^{[1
]}

Reilly, John ^{[1
]}

Wagner, Trixie ^{[1
]}

Brocklehurst, Cara E. ^{[1
]}

Wyler, Rene ^{[1
]}

Dunstan, David ^{[2
,5
]}

Marziale, Alexander N. ^{[1
,6
]}

机构：

[1] Novartis Pharm AG, Novartis Inst Biomed Res, Global Discovery Chem, Fabrikstr 1, CH-4056 Basel, Switzerland

[2] Novartis Pharm AG, Novartis Inst Biomed Res, Global Discovery Chem, Cambridge, MA 02139 USA

[3] Virscidian Inc, Cary, NC 27511 USA

[4] Valo Hlth, 75 Hayden Ave, Lexington, MA 02421 USA

[5] Relay Therapeut, 399 Binney St, Cambridge, MA 02139 USA

[6] AstraZeneca, Pepparedsleden 1, S-43183 Molndal, Sweden

来源：

SLAS TECHNOLOGY | 2022年 / 27卷 / 06期

关键词：

High-throughput purification; Laboratory automation; Library purification; Microscale libraries; Analytical chemistry automation; PERFORMANCE LIQUID-CHROMATOGRAPHY; CHARGED AEROSOL DETECTION; UNIVERSAL DETECTORS; CHEMISTRY; GENERATION; INHIBITORS; PLATFORM; IDENTIFICATION; IMPURITIES; MODULATORS;

D O I：

10.1016/j.slast.2022.08.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We herein report the development of an automation platform for rapid purification and quantification of chem-ical libraries including reformatting of chemical matter to 10 mM DMSO stock solutions. This fully integrated workflow features tailored conditions for preparative reversed-phase (RP) HPLC-MS on microscale based on ana-lytical data, online fraction QC and CAD-based quantification as well as automated reformatting to enable rapid purification of chemical libraries. This automated workflow is entirely solution-based, eliminating the need to weigh or handle solids. This increases process efficiency and creates a link between high-throughput synthesis and profiling of novel chemical matter with respect to biological and physicochemical properties in relevant assays.

引用

页码：350 / 360

页数：11

共 50 条

[1] Automated high-throughput protein purification using an AKTApurifier and a CETAC autosampler
Yoo, Daniel
Provchy, Justin
Park, Cynthia
Schulz, Craig
Walker, Kenneth
JOURNAL OF CHROMATOGRAPHY A, 2014, 1344 : 23 - 30
[2] Microscale Adaptation of In Vitro Transcription/Translation for High-Throughput Screening of Natural Product Extract Libraries
Lowell, Andrew N.
Santoro, Nicholas
Swaney, Steven M.
McQuade, Thomas J.
Schultz, Pamela J.
Larsen, Martha J.
Sherman, David H.
CHEMICAL BIOLOGY & DRUG DESIGN, 2015, 86 (06) : 1331 - 1338
[3] High-Throughput Purification Platform in Support of Drug Discovery
Liu, Min
Chen, Kuanchang
Christian, Denny
Fatima, Tazeen
Pissamitski, Natalya
Streckfuss, Eric
Zhang, Chaowei
Xia, Lei
Borges, Scott
Shi, Zhicai
Vachal, Petr
Tata, James
Athanasopoulos, John
ACS COMBINATORIAL SCIENCE, 2012, 14 (01) : 51 - 59
[4] Cytotoxic Profiling of Annotated and Diverse Chemical Libraries Using Quantitative High-Throughput Screening
Lee, Olivia W.
Austin, Shelley
Gamma, Madison
Cheff, Dorian M.
Lee, Tobie D.
Wilson, Kelli M.
Johnson, Joseph
Travers, Jameson
Braisted, John C.
Guha, Rajarshi
Klumpp-Thomas, Carleen
Shen, Min
Hall, Matthew D.
SLAS DISCOVERY, 2020, 25 (01) : 9 - 20
[5] Automated high-throughput DNA synthesis and assembly
Ma, Yuxin
Zhang, Zhaoyang
Jia, Bin
Yuan, Yingjin
HELIYON, 2024, 10 (06)
[6] Data processing for high-throughput mass spectrometry in drug discovery
Liu, Chang
Zhang, Hui
EXPERT OPINION ON DRUG DISCOVERY, 2024, 19 (07) : 815 - 825
[7] An Automated Purification Workflow Coupled with Material-Sparing High-Throughput 1H NMR for Parallel Medicinal Chemistry
Bellenger, Justin
Koos, Martin R. M.
Avery, Melissa
Bundesmann, Mark
Ciszewski, Gregory
Khunte, Bhagyashree
Leverett, Carolyn
Ostner, Gregory
Ryder, Tim F.
Farley, Kathleen A.
ACS MEDICINAL CHEMISTRY LETTERS, 2024, 15 (09): : 1635 - 1644
[8] Automated Enrichment of Phosphotyrosine Peptides for High-Throughput Proteomics
Chang, Alexis
Leutert, Mario
Rodriguez-Mias, Ricard A.
Villen, Judit
JOURNAL OF PROTEOME RESEARCH, 2023, 22 (06) : 1868 - 1880
[9] Integrated Qualitative Strategy for High-throughput Targeted Filtering of Emodin Metabolites
Cai Fang
Ren Fan-Dung
Ren Da-Bing
Liu Shao
Yi Lun-Zhao
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2019, 47 (08) : 1212 - 1218
[10] The Developing Zebrafish (Danio rerio): A Vertebrate Model for High-throughput Screening of Chemical Libraries
Lessman, Charles A.
BIRTH DEFECTS RESEARCH PART C-EMBRYO TODAY-REVIEWS, 2011, 93 (03) : 268 - 280

← 1 2 3 4 5 →