Proteins as micro viscosimeters:: Brownian motion revisited

被引：42

作者：

Lavalette, Daniel

Hink, Mark A.

Tourbez, Martine

Tetreau, Catherine

Visser, Antonie J.

机构：

[1] Ctr Univ Orsay, Inst Curie Rech, F-91405 Orsay, France

[2] Univ Wageningen, MicroSpect Ctr, Biochem Lab, NL-6703 HA Wageningen, Netherlands

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2006年 / 35卷 / 06期

关键词：

Brownian motion; microviscosity; microscopic hydrodynamics; diffusion in the cell;

D O I：

10.1007/s00249-006-0060-z

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Translational and rotational diffusion coefficients of proteins in solution strongly deviate from the Stokes-Einstein laws when the ambient viscosity is induced by macromolecular co-solutes rather than by a solvent of negligible size as was assumed by A. Einstein one century ago for deriving the laws of Brownian motion and diffusion. Rotational and translational motions experience different micro viscosities and both become a function of the size ratio of protein and macromolecular co-solute. Possible consequences upon fluorescence spectroscopy observations of diffusing proteins within living cells are discussed.

引用

页码：517 / 522

页数：6

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