Performance of free β-human chorionic gonadotrophin (free β-hCG) and pregnancy associated plasma protein-A (PAPP-A) analysis between Delfia Xpress and AutoDelfia systems in The Netherlands

被引：11
|
作者：
Linskens, Ingeborg H. [2 ]
Levitus, Marieke [1 ]
Frans, Anneke [1 ]
Schielen, Peter C. J. I. [3 ]
van Vugt, John M. G. [2 ]
Blankenstein, Marinus A. [1 ]
Dijstelbloem, Hilde M. [1 ]
机构：
[1] Vrije Univ Amsterdam, Med Ctr, Dept Clin Chem, NL-1007 MB Amsterdam, Netherlands
[2] Vrije Univ Amsterdam, Med Ctr, Dept Obstet & Gynecol, NL-1007 MB Amsterdam, Netherlands
[3] Natl Inst Publ Hlth & Environm RIVM, Bilthoven, Netherlands
来源：
CLINICAL CHEMISTRY AND LABORATORY MEDICINE | 2009年 / 47卷 / 02期
关键词：
AutoDelfia; Delfia Xpress; first trimester Down syndrome screening; free beta-human chorionic gonadotrophin; pregnancy associated plasma protein-A; FETAL NUCHAL TRANSLUCENCY; MAJOR BASIC-PROTEIN; DOWN-SYNDROME; 1ST TRIMESTER; SERUM; SUBUNIT; MARKERS; RISK; TRISOMY-21; STABILITY;
D O I：
10.1515/CCLM.2009.042
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background: The VU University Medical Center (VUmc) was the first hospital in the Netherlands to introduce the Delfia Xpress for the analysis of free beta-human chorionic gonadotrophin (beta-hCG) and pregnancy associated plasma protein-A (PAPP-A) in the first trimester screening program for Down syndrome. Since then, others have implemented this system. In this study, we tested the equality of measurements for free beta-hCG and PAPP-A between Delfia Xpress systems and one AutoDelfia system. Methods: A total of 40 serum samples were aliquoted and stored at - 20 degrees C. Samples were analyzed by six Delfia Xpress systems and one AutoDelfia system over a time period of 2 years. Results: The relationships between free beta-hCG and PAPP-A were excellent for the different Delfia Xpress systems (r > 0.99, p < 0.0001). For PAPP-A, the agreement between the main system at VUmc and five other systems was linear with slopes between 0.99 and 1.06. Similarly, agreement for free beta-hCG was linear with slopes between 0.99 and 1.09. Likewise, agreement for PAPP-A and free beta-hCG was excellent for the AutoDelfia vs. the main Delfia Xpress at the VUmc (r > 0.99, p < 0.0001). For both PAPP-A and free beta-hCG, the relationships were linear with slopes of 1.08 and 1.07. Conclusions: We demonstrate an excellent agreement for the analysis of PAPP-A and free beta-hCG between Delfia Xpress systems and one AutoDelfia system.
引用
收藏
页码：222 / 226
页数：5
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