Construction of epidermal growth factor fusion protein with cell adhesive activity

被引：45
|
作者：
Elloumi, I [1 ]
Kobayashi, R [1 ]
Funabashi, H [1 ]
Mie, M [1 ]
Kobatake, E [1 ]
机构：
[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat, Midori Ku, Yokohama, Kanagawa 2268501, Japan
来源：
BIOMATERIALS | 2006年 / 27卷 / 18期
关键词：
epidermal growth factor; RGD; hydrophobic structure; fusion protein; cell adhesion;
D O I：
10.1016/j.biomaterials.2006.02.003
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
A novel protein for controlling cellular functions was constructed by combining functional units of various proteins. The Arg-Gly-Asp (RGD) sequence functioning as a cell adhesive function, an epidermal growth factor (EGF) as a cell growth function, and a hydrophobic sequence (E12) as an efficient assembling function, were combined and incorporated into one molecule. The fusion protein, designated ERE-EGF, was produced in Escherichia coli and purified with affinity chromatography using a His-tag. The ERE-EGF coated on an unmodified hydrophobic Surface of a cell-culture plate (through the hydrophobic E12 moiety) retained both cell adhesive activity (through the RGD sequence) and cell growth activity (through the EGF moiety). (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：3451 / 3458
页数：8
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