Self-assembled films containing crude extract of avocado as a source of tyrosinase for monophenol detection

被引:4
作者
Vieira, Nirton C. S. [1 ]
Ferreira, Reginaldo A. [2 ]
Rodrigues, Valquiria da Cruz [1 ]
Guimaraes, Francisco E. G. [1 ]
de Queiroz, Alvaro A. A. [2 ]
机构
[1] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
[2] Univ Fed Itajuba, Ctr Estudos & Inovacao Mat Biofuncionais Avancado, BR-37500903 Itajuba, MG, Brazil
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2013年 / 33卷 / 07期
关键词
Crude extract; Tyrosinase; Biosensor; Monophenol; Layer by layer; FIELD-EFFECT TRANSISTORS; PHENOLS DETERMINATION; BIOSENSOR; WATER;
D O I
10.1016/j.msec.2013.05.028
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
This paper reports on the use of the crude extract of avocado (CEA) fruit (Persea americana) as a source of tyrosinase enzyme. CEA was immobilized via layer by layer (LbL) technique onto indium tin oxide (ITO) substrates and applied in the detection of monophenol using a potentiometric biosensor. Poly(propylene imine) dendrimer of generation 3 (PPI-G3) was used as a counter ion in the layer by layer process due to its highly porous structure and functional groups suitable for enzyme linkage. After the immobilization of the crude CEA as multilayered. films, standard samples of monophenol were detected in the 0.25-4.00 mM linear range with approximately 28 mV mM(-1) of sensitivity. This sensitivity is 14 times higher than the values found in the literature for a similar system. The results show that it is possible to obtain efficient and low-cost biosensors for monophenol detection using potentiometric transducers and alternative sources of enzymes without purification. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:3899 / 3902
页数:4
相关论文
共 22 条
[1]   Development of a potentiometric catechol biosensor by entrapment of tyrosinase within polypyrrole film [J].
Ameer, Qaisar ;
Adeloju, Samuel B. .
SENSORS AND ACTUATORS B-CHEMICAL, 2009, 140 (01) :5-11
[2]   Development of tyrosinase biosensor based on pH-sensitive field-effect transistors for phenols determination in water solutions [J].
Anh, TM ;
Dzyadevych, SV ;
Soldatkin, AP ;
Chien, ND ;
Jaffrezic-Renault, N ;
Chovelon, JM .
TALANTA, 2002, 56 (04) :627-634
[3]   Thirty years of ISFETOLOGY - What happened in the past 30 years and what may happen in the next 30 years [J].
Bergveld, P .
SENSORS AND ACTUATORS B-CHEMICAL, 2003, 88 (01) :1-20
[4]   Study on extended gate field effect transistor with tin oxide sensing membrane [J].
Chi, LL ;
Chou, JC ;
Chung, WY ;
Sun, TP ;
Hsiung, SK .
MATERIALS CHEMISTRY AND PHYSICS, 2000, 63 (01) :19-23
[5]   CHROMATOGRAPHIC DETERMINATION OF PHENOLS IN WATER [J].
CHRISWELL, CD ;
CHANG, RC ;
FRITZ, JS .
ANALYTICAL CHEMISTRY, 1975, 47 (08) :1325-1329
[6]   THE DETERMINATION OF PHENOLS BY CHROMATOGRAPHY AND SPECTROPHOTOMETRY OF THEIR METHYL ETHERS .1. ULTRAVIOLET ABSORPTION SPECTRA OF METHYL ARYL ETHERS IN CYCLOHEXANE [J].
COMMINS, BT ;
LINDSEY, AJ .
ANALYTICA CHIMICA ACTA, 1956, 15 (05) :446-450
[7]   Fuzzy nanoassemblies: Toward layered polymeric multicomposites [J].
Decher, G .
SCIENCE, 1997, 277 (5330) :1232-1237
[8]   Development of enzyme biosensor based on pH-sensitive field-effect transistors for detection of phenolic compounds [J].
Dzyadevych, SV ;
Anh, TM ;
Soldatkin, AP ;
Chien, ND ;
Jaffrezic-Renault, N ;
Chovelon, JM .
BIOELECTROCHEMISTRY, 2002, 55 (1-2) :79-81
[9]   Immobilization of Poly(propylene imine) Dendrimer/Nickel Phtalocyanine as Nanostructured Multilayer Films To Be Used as Gate Membranes for SEGFET pH Sensors [J].
Fernandes, Edson G. R. ;
Vieira, Nirton C. S. ;
de Queiroz, Alvaro A. A. ;
Guimaraes, Francisco E. G. ;
Zucolotto, Valtencir .
JOURNAL OF PHYSICAL CHEMISTRY C, 2010, 114 (14) :6478-6483
[10]   Enzyme-mediated amperometric biosensors prepared with the Layer-by-Layer (LbL) adsorption technique [J].
Ferreira, M ;
Fiorito, PA ;
Oliveira, ON ;
de Torresi, SIC .
BIOSENSORS & BIOELECTRONICS, 2004, 19 (12) :1611-1615