Structural Characterization and In Vivo Anti-Inflammatory Activity of Fucoidan from Cystoseira crinita (Desf.) Borry

被引：26

作者：

Apostolova, Elisaveta ^{[1
]}

Lukova, Paolina ^{[2
]}

Baldzhieva, Alexandra ^{[3
,4
]}

Delattre, Cedric ^{[5
,6
]}

Molinie, Roland ^{[7
]}

Petit, Emmanuel ^{[7
]}

Elboutachfaiti, Redouan ^{[7
]}

Nikolova, Mariana ^{[8
]}

Iliev, Ilia ^{[8
]}

Murdjeva, Marianna ^{[3
,4
]}

Kokova, Vesela ^{[1
]}

机构：

[1] Med Univ Plovdiv, Fac Pharm, Dept Pharmacol Toxicol & Pharmacotherapy, Vasil Aprilov Str 15A, Plovdiv 4002, Bulgaria

[2] Med Univ Plovdiv, Fac Pharm, Dept Pharmacognosy & Pharmaceut Chem, Vasil Aprilov Str 15A, Plovdiv 4002, Bulgaria

[3] Med Univ Plovdiv, Fac Pharm, Dept Microbiol & Immunol, Vasil Aprilov Str 15A, Plovdiv 4002, Bulgaria

[4] Med Univ Plovdiv, Res Inst, Vasil Aprilov Str 15A, Plovdiv 4002, Bulgaria

[5] Univ Clermont Auvergne, Inst Pascal, CNRS, Clermont Auvergne INP, F-63000 Clermont Ferrand, France

[6] Inst Univ France IUF, 1 Rue Descartes, F-75005 Paris, France

[7] Univ Picardie Jules Verne, IUT Amiens, Biol Plantes & Innovat BIOPI, UMRT INRAE 1158 BioEcoAgro, Ave Fac, F-80025 Amiens, France

[8] Plovdiv Univ Paisii Hilendarski, Fac Biol, Dept Biochem & Microbiol, Tsar Asen Str 24, Plovdiv 4000, Bulgaria

来源：

MARINE DRUGS | 2022年 / 20卷 / 11期

关键词：

fucoidan; Cystoseira crinita; TNF-alpha; IL-1; beta; peritonitis; rat paw edema; cytokines; anti-inflammatory effect; WATER-SOLUBLE POLYSACCHARIDES; BROWN SEAWEEDS; SULFATED POLYSACCHARIDES; ANTIOXIDANT; ANTICOAGULANT; EXTRACTION; FUCANS; VITRO; MACROPHAGES; COLITIS;

D O I：

10.3390/md20110714

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The aim of this study was to evaluate the effects of fucoidan isolated from C. crinita on histamine-induced paw inflammation in rats, and on the serum levels of TNF-alpha, IL-1 beta, IL-6, and IL-10 in rats during systemic inflammation response. The levels of TNF-alpha in a model of acute peritonitis in rats were also investigated. The isolated crude fucoidan was identified as a sulfated xylogalactofucan with high, medium, and low molecular weight fractions and a content of fucose of 39.74%, xylose of 20.75%, and galactose of 15.51%. Fucoidan from C. crinita showed better anti-inflammatory effects in the rat paw edema model, and this effect was present during all stages of the experiment. When compared to controls, a commercial fucoidan from F. vesiculosus, the results also displayed anti-inflammatory activity on the 60th, 90th, and 120th minute of the experiment. A significant decrease in serum levels of IL-1 beta in rats treated with both doses of C. crinita fucoidan was observed in comparison to controls, whereas TNF-alpha concentrations were reduced only in the group treated with fucoidan from C. crinita at the dose of 25 mg/kg bw. In the model of carrageenan-induced peritonitis, we observed a tendency of decrease in the levels of the pro-inflammatory cytokine TNF-alpha in peritoneal fluid after a single dose of C. crinita fucoidan, but this did not reach the statistical significance margin. Single doses of C. crinita fucoidan did not alter serum levels of the anti-inflammatory cytokine IL-10 in animals with lipopolysaccharide-induced systemic inflammation.

引用

页数：20

共 69 条

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