The optical cell rotator

被引：103

作者：

Kreysing, Moritz K. ^{[1
,2
]}

Kiessling, Tobias ^{[1
]}

Fritsch, Anatol ^{[1
]}

Dietrich, Christian ^{[3
]}

Guck, Jochen R. ^{[1
,2
]}

Kaes, Josef A. ^{[1
]}

机构：

[1] Univ Leipzig, Dept Expt Phys 1, Leipzig, Germany

[2] Univ Cambridge, Cavendish Lab, Dept Phys, Cambridge CB3 0HE, England

[3] Carl Zeiss MicroImaging GmbH, Jena, Germany

来源：

OPTICS EXPRESS | 2008年 / 16卷 / 21期

关键词：

D O I：

10.1364/OE.16.016984

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The optical cell rotator (OCR) is a modified dual-beam laser trap for the holding and controlled rotation of suspended dielectric microparticles, such as cells. In contrast to optical tweezers, OCR uses two counter-propagating divergent laser beams, which are shaped and delivered by optical fibers. The rotation of a trapped specimen is carried out by the rotation of a dual-mode fiber, emitting an asymmetric laser beam. Experiments were performed on human erythrocytes, promyelocytic leukemia cells (HL60), and cell clusters (MCF-7). Since OCR permits the rotation of cells around an axis perpendicular to the optical axis of any microscope and is fully decoupled from imaging optics, it could be a suitable and expedient tool for tomographic microscopy. (C) 2008 Optical Society of America

引用

页码：16984 / 16992

页数：9

共 33 条

[21] Axial tomographic confocal fluorescence microscopy
Heintzmann, R
Cremer, C
[J]. JOURNAL OF MICROSCOPY-OXFORD, 2002, 206 (01): : 7 - 23
[22] Optical sectioning deep inside live embryos by selective plane illumination microscopy
Huisken, J
Swoger, J
Del Bene, F
Wittbrodt, J
Stelzer, EHK
[J]. SCIENCE, 2004, 305 (5686) : 1007 - 1009
[23] Cell damage in near-infrared multimode optical traps as a result of multiphoton absorption
Konig, K
Liang, H
Berns, MW
Tromberg, BJ
[J]. OPTICS LETTERS, 1996, 21 (14) : 1090 - 1092
[24] KREYSING M, 2007, Patent No. 1935498
[25] Rotational control within optical tweezers by use of a rotating aperture
O'Neil, AT
Padgett, MJ
[J]. OPTICS LETTERS, 2002, 27 (09) : 743 - 745
[26] Controlled rotation of optically trapped microscopic particles
Paterson, L
MacDonald, MP
Arlt, J
Sibbett, W
Bryant, PE
Dholakia, K
[J]. SCIENCE, 2001, 292 (5518) : 912 - 914
[27] Renaud Olivier, 2008, Biotechnology Journal, V3, P53, DOI 10.1002/biot.200700188
[28] SYNTHESIS OF INTENSITY PATTERNS IN FEW-MODE OPTICAL FIBERS
SAFAAIJAZI, A
MCKEEMAN, JC
[J]. JOURNAL OF LIGHTWAVE TECHNOLOGY, 1991, 9 (09) : 1047 - 1052
[29] OPTICAL TRAPPING AND ROTATIONAL MANIPULATION OF MICROSCOPIC PARTICLES AND BIOLOGICAL CELLS USING HIGHER-ORDER MODE ND-YAG LASER-BEAMS
SATO, S
ISHIGURE, M
INABA, H
[J]. ELECTRONICS LETTERS, 1991, 27 (20) : 1831 - 1832
[30] TILTED VIEW RECONSTRUCTION IN OPTICAL MICROSCOPY - 3-DIMENSIONAL RECONSTRUCTION OF DROSOPHILA-MELANOGASTER EMBRYO NUCLEI
SHAW, PJ
AGARD, DA
HIRAOKA, Y
SEDAT, JW
[J]. BIOPHYSICAL JOURNAL, 1989, 55 (01) : 101 - 110

← 1 2 3 4 →