The optical cell rotator

被引：105

作者：

Kreysing, Moritz K. ^{[1
,2
]}

Kiessling, Tobias ^{[1
]}

Fritsch, Anatol ^{[1
]}

Dietrich, Christian ^{[3
]}

Guck, Jochen R. ^{[1
,2
]}

Kaes, Josef A. ^{[1
]}

机构：

[1] Univ Leipzig, Dept Expt Phys 1, Leipzig, Germany

[2] Univ Cambridge, Cavendish Lab, Dept Phys, Cambridge CB3 0HE, England

[3] Carl Zeiss MicroImaging GmbH, Jena, Germany

来源：

OPTICS EXPRESS | 2008年 / 16卷 / 21期

关键词：

D O I：

10.1364/OE.16.016984

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The optical cell rotator (OCR) is a modified dual-beam laser trap for the holding and controlled rotation of suspended dielectric microparticles, such as cells. In contrast to optical tweezers, OCR uses two counter-propagating divergent laser beams, which are shaped and delivered by optical fibers. The rotation of a trapped specimen is carried out by the rotation of a dual-mode fiber, emitting an asymmetric laser beam. Experiments were performed on human erythrocytes, promyelocytic leukemia cells (HL60), and cell clusters (MCF-7). Since OCR permits the rotation of cells around an axis perpendicular to the optical axis of any microscope and is fully decoupled from imaging optics, it could be a suitable and expedient tool for tomographic microscopy. (C) 2008 Optical Society of America

引用

页码：16984 / 16992

页数：9

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