Cultured rat microglia express functional β-chemokine receptors

被引：103
作者：
Boddeke, EWGM
Meigel, I
Frentzel, S
Gourmala, NG
Harrison, JK
Buttini, M
Spleiss, O
Gebicke-Härter, P
机构：
[1] Univ Groningen, Dept Med Physiol, NL-9712 KZ Groningen, Netherlands
[2] Novartis Pharma, NS Res, CH-4002 Basel, Switzerland
[3] Univ Florida, Dept Neurosci, Gainesville, FL 32610 USA
[4] Univ Calif San Francisco, Gladstone Mol Neurobiol Program, San Francisco, CA 94141 USA
[5] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94141 USA
[6] Univ Freiburg, Dept Psychiat, D-79104 Freiburg, Germany
来源：
JOURNAL OF NEUROIMMUNOLOGY | 1999年 / 98卷 / 02期
关键词：
chemokines; signal transduction; lipopolysaccharide; neuroimmunology;
D O I：
10.1016/S0165-5728(99)00096-X
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We have investigated the functional expression of the beta-chemokine receptors CCR1 to 5 in cultured rat microglia. RT-PCR analysis revealed constitutive expression of CCR1, CCR2 and CCR5 mRNA. The beta-chemokines MCP-1 (1-30 nM) as well as RANTES and MIP-1 alpha (100-1000 nM) evoked calcium transients in control and LPS-treated microglia. Whereas, the response to MCP-1 was dependent on extracellular calcium the response to RANTES was not. The effect of MCP-1 but not that of RANTES was inhibited by the calcium-induced calcium release inhibitor ryanodine. Calcium responses to MCP-1- and RANTES were observed in distinct populations of microglia. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：176 / 184
页数：9
相关论文
共 39 条
[1]  
AGUZZI A, 1990, CURRENT PROTOCOLS S, V9, P1
[2]   Chemokine gene expression in the brains of mice with lymphocytic choriomeningitis [J].
Asensio, VC ;
Campbell, IL .
JOURNAL OF VIROLOGY, 1997, 71 (10) :7832-7840
[3]   Identification of CCR6, the specific receptor for a novel lymphocyte-directed CC chemokine LARC [J].
Baba, M ;
Imai, T ;
Nishimura, M ;
Kakizaki, M ;
Takagi, S ;
Hieshima, K ;
Nomiyama, H ;
Yoshie, O .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (23) :14893-14898
[4]   Chemokines and leukocyte traffic [J].
Baggiolini, M .
NATURE, 1998, 392 (6676) :565-568
[5]   Human chemokines: An update [J].
Baggiolini, M ;
Dewald, B ;
Moser, B .
ANNUAL REVIEW OF IMMUNOLOGY, 1997, 15 :675-705
[6]   MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF 2 MONOCYTE CHEMOATTRACTANT PROTEIN-1 RECEPTORS REVEALS ALTERNATIVE SPLICING OF THE CARBOXYL-TERMINAL TAILS [J].
CHARO, IF ;
MYERS, SJ ;
HERMAN, A ;
FRANCI, C ;
CONNOLLY, AJ ;
COUGHLIN, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (07) :2752-2756
[7]   Cloning and functional expression of CC CKR5, a human monocyte CC chemokine receptor selective for MIP-1 alpha, MIP-1 beta, and RANTES [J].
Combadiere, C ;
Ahuja, SK ;
Tiffany, HL ;
Murphy, PM .
JOURNAL OF LEUKOCYTE BIOLOGY, 1996, 60 (01) :147-152
[8]   HUMAN ASTROCYTOMAS AND GLIOBLASTOMAS EXPRESS MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) IN-VIVO AND IN-VITRO [J].
DESBAILLETS, I ;
TADA, M ;
DETRIBOLET, N ;
DISERENS, AC ;
HAMOU, MF ;
VAN MEIR, EG .
INTERNATIONAL JOURNAL OF CANCER, 1994, 58 (02) :240-247
[9]  
Dubois PM, 1996, J IMMUNOL, V156, P1356
[10]  
FRANCI C, 1995, J IMMUNOL, V154, P6511
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