Preparation of a Near-Infrared Fluorescent Probe Based on IR-780 for Highly Selective and Sensitive Detection of Bisulfite-Sulfite in Food, Living Cells, and Mice

A near-infrared fluorescent probe was designed and synthesized for the detection of bisulfite-sulfite in food, living HeLa cells, and mice. The probe is a stable hemicyanine skeleton based on IR-780, which produces a distinct color and fluorescence change before and after reaction with bisulfite and shows excellent analytical performance, such as high sensitivity with a detection limit of 0.37 mu M and high selectivity toward bisulfite over other substances. More importantly, the probe determined bisulfite in real food samples (crystal sugar and red wine) with superior recoveries (96.7-106.1%) and has been successfully used to monitor bisulfite in living HeLa cells. Notably, the probe was applied to image bisulfite in BALB/c mice in vivo for the first time. Owing to its biocompatibility, this probe provides a desired method to study the metabolism of bisulfite in cells and shows substantial potential usage in other biosystems and in vivo imaging studies.