One-step separation system for the main phospholipids, glycolipids, and phenolics by normal phase HPLC. Application to polar lipid extracts from olive and sunflower oils

A new normal phase high performance liquid chromatographic system has been developed that allows efficient separation or several polar lipids, such as phospholipids, glycolipids, and phenolics. By this method, hesperidin, naringin, cerebrosides, phosphatidylcholine, sphingomyelin, platelet activating factor, lysophosphatidylcholine, digalactosyldiglycerides, and phosphatidylethanolamine are separated within 60 min. A gradient elution with acetonitrile/methanol, methanol, and water was performed with a normal phase aminopropyl-modified silica gel HPLC column. The eluted lipids were monitored by UV detection. In addition, in this study, a successful application of the new method is represented for the polar lipids of olive oil and sunflower, which are also identified with chemical determinations.