Raman Microscopy and Associated Techniques for Label-Free Imaging of Cancer Tissue

被引：12

作者：

Downes, Andrew ^{[1
]}

机构：

[1] Univ Edinburgh, Inst Bioengn, Edinburgh EH9 3JL, Midlothian, Scotland

来源：

APPLIED SPECTROSCOPY REVIEWS | 2015年 / 50卷 / 08期

关键词：

Biological; Raman spectroscopy; analytical; BREAST-CANCER; HIGH-SENSITIVITY; SCATTERING; SPECTROSCOPY; DISCRIMINATION; IDENTIFICATION; ALGORITHM; MOLECULES; DIAGNOSIS; SURGERY;

D O I：

10.1080/05704928.2015.1052817

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Raman spectroscopy can identify cancerous from healthy tissue, with a chemical analysis from the measurement of vibrational bond frequencies. However, to detect small tumors a form of Raman imaging is required. Such imaging-by acquiring a Raman spectrum at each imaging pixel-can detect tumors but is rather slow. Multiphoton versions of Raman-anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering (SRS) microscopy-offer similar accuracies in identifying cancerous tissue and tumor margins but with a far higher speed, which is beneficial for diagnosis of small tumors in tissue. SRS microscopy can also be used to image extrinsic molecules in living cells, such as anti-cancer drugs at typical concentrations.

引用

页码：641 / 653

页数：13

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