Using three-dimensional acinar structures for molecular and cell biological assays

被引：40

作者：

Xiang, B ^{[1
]}

Muthuswamy, SK ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

来源：

METHODS IN ENZYMOLOGY, VOL 406, REGULATORS AND EFFECTORS OF SMALL GTPASES: RHO FAMILY | 2006年 / 406卷

关键词：

D O I：

10.1016/S0076-6879(06)06054-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Epithelial cells grown in three-dimensional matrix allows one to investigate biological processes in a setting that is closer to in vivo conditions than those obtained by growing cells on plastic culture dishes. Here we outline procedures that allow one to investigate molecular mechanisms that regulate formation and disruption of three-dimensional epithelial structures.

引用

页码：692 / 701

页数：10

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