Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis

被引：12
作者：
Caleffi-Ferracioli, Katiany R. [1 ]
Cardoso, Rosilene F. [1 ]
de Souza, Joao V. P. [1 ]
Murase, Leticia S. [1 ]
Canezin, Pedro H. [1 ]
Scodro, Regiane B. L. [1 ]
Siqueira, Vera L. D. [1 ]
Pavan, Fernando R. [2 ]
机构：
[1] Univ Estadual Maringa, Dept Clin Anal & Biomed, Lab Med Bacteriol, Maringa, Parana, Brazil
[2] Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Biol Sci, Sao Paulo, Brazil
来源：
FUTURE MICROBIOLOGY | 2019年 / 14卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
efflux pump inhibitor; gene regulation; multidrug transporters; Mycobacterium tuberculosis; real-time PCR; resistance; rifampicin; synergism; tuberculosis; verapamil; ABC EFFLUX PUMP; DRUG-RESISTANCE; GENE-EXPRESSION; MECHANISMS; MUTATIONS;
D O I：
10.2217/fmb-2018-0277
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis. Materials & methods: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. Results: VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate. Conclusion: Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in M. tuberculosis.
引用
收藏
页码：185 / 194
页数：10
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