Modulatory effects of verapamil in rifampicin activity against Mycobacterium tuberculosis

被引：10
|
作者：
Caleffi-Ferracioli, Katiany R. [1 ]
Cardoso, Rosilene F. [1 ]
de Souza, Joao V. P. [1 ]
Murase, Leticia S. [1 ]
Canezin, Pedro H. [1 ]
Scodro, Regiane B. L. [1 ]
Siqueira, Vera L. D. [1 ]
Pavan, Fernando R. [2 ]
机构：
[1] Univ Estadual Maringa, Dept Clin Anal & Biomed, Lab Med Bacteriol, Maringa, Parana, Brazil
[2] Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Biol Sci, Sao Paulo, Brazil
来源：
FUTURE MICROBIOLOGY | 2019年 / 14卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
efflux pump inhibitor; gene regulation; multidrug transporters; Mycobacterium tuberculosis; real-time PCR; resistance; rifampicin; synergism; tuberculosis; verapamil; ABC EFFLUX PUMP; DRUG-RESISTANCE; GENE-EXPRESSION; MECHANISMS; MUTATIONS;
D O I：
10.2217/fmb-2018-0277
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis. Materials & methods: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. Results: VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate. Conclusion: Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in M. tuberculosis.
引用
收藏
页码：185 / 194
页数：10
相关论文
共 50 条
  • [1] Isoniazid and verapamil modulatory activity and efflux pump gene expression in Mycobacterium tuberculosis
    de Souza, J. V. P.
    Murase, L. S.
    Caleffi-Ferracioli, K. R.
    Palomo, C. T.
    Scodro, R. B. de Lima
    Siqueira, V. L. D.
    Pavan, F. R.
    Cardoso, R. F.
    INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 2020, 24 (06) : 591 - +
  • [2] In Vitro Activity of Rifampicin and Verapamil Combination in Multidrug-Resistant Mycobacterium tuberculosis
    Demitto, Fernanda de Oliveira
    Ribeiro do Amaral, Renata Claro
    Maltempe, Flaviane Granero
    Dias Siqueira, Vera Lucia
    de Lima Scodro, Regiane Bertin
    Lopes, Mariana Aparecida
    Caleffi-Ferracioli, Katiany R.
    Canezin, Pedro Henrique
    Cardoso, Rosilene Fressatti
    PLOS ONE, 2015, 10 (02):
  • [3] Activity of rifampicin and linezolid combination in Mycobacterium tuberculosis
    Maltempe, Flaviane Granero
    Caleffi-Ferracioli, Katiany Rizzieri
    Ribeiro do Amaral, Renata Claro
    Demitto, Fernanda de Oliveira
    Dias Siqueira, Vera Lucia
    de Lima Scodro, Regiane Bertin
    Hirata, Mario Hiroyuki
    Pavan, Fernando Rogerio
    Cardoso, Rosilene Fressatti
    TUBERCULOSIS, 2017, 104 : 24 - 29
  • [4] Molecular mechanism of rifampicin and isoniazid resistance in Mycobacterium tuberculosis from Bangladesh
    Rahim, Zeaur
    Nakajima, Chie
    Raqib, Rubhana
    Zaman, Khalequ
    Endtz, Hubert P.
    van der Zanden, Adri G. M.
    Suzuki, Yasuhiko
    TUBERCULOSIS, 2012, 92 (06) : 529 - 534
  • [5] A highly rifampicin resistant Mycobacterium tuberculosis strain emerging in Southern Brazil
    Rossetti, Maria Lucia
    Almeida da Silva, Pedro Eduardo
    Salvato, Richard Steiner
    Reis, Ana Julia
    Schiefelbein, Sun Hee
    von Groll, Andrea
    Barcellos, Regina Bones
    Maschmann, Raquel
    Esteves, Leonardo Souza
    Spies, Fernanda
    Trespach, Rubia Raubach
    Dalla Costa, Elis Regina
    Neubauer de Amorim, Hermes Luis
    TUBERCULOSIS, 2020, 125
  • [6] GeneXpert Mycobacterium tuberculosis/rifampicin assay for molecular epidemiology of rifampicin-Resistant Mycobacterium tuberculosis in an Urban Setting of Banten province, Indonesia
    Christopher, Paulus Mario
    Cucunawangsih
    Widysanto, Allen
    INTERNATIONAL JOURNAL OF MYCOBACTERIOLOGY, 2019, 8 (04) : 351 - 358
  • [7] Rifampicin-induced transcriptome response in rifampicin-resistant Mycobacterium tuberculosis
    de Knegt, Gerjo J.
    Bruning, Oskar
    ten Kate, Marian T.
    de Jong, Mark
    van Belkum, Alex
    Endtz, Hubert P.
    Breit, Timo M.
    Bakker-Woudenberg, Irma A. J. M.
    de Steenwinkel, Jurriaan E. M.
    TUBERCULOSIS, 2013, 93 (01) : 96 - 101
  • [8] Synthesis of new verapamil analogues and their evaluation in combination with rifampicin against Mycobacterium tuberculosis and molecular docking studies in the binding site of efflux protein Rv1258c
    Singh, Kawaljit
    Kumar, Malkeet
    Pavadai, Elumalai
    Naran, Krupa
    Warner, Digby F.
    Ruminski, Peter G.
    Chibale, Kelly
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2014, 24 (14) : 2985 - 2990
  • [9] Evaluation of Mycobacterium tuberculosis rpoB for detecting rifampicin resistance
    Koentjoro, Maharani Pertiwi
    Prasetyo, Endry Nugroho
    RESEARCH JOURNAL OF BIOTECHNOLOGY, 2021, 16 (01): : 129 - 135
  • [10] Morphological changes and differentially expressed efflux pump genes in Mycobacterium tuberculosis exposed to a rifampicin and verapamil combination
    Caleffi-Ferracioli, Katiany R.
    Amaral, Renata Claro R.
    Demitto, Fernanda O.
    Maltempe, Flaviane G.
    Canezin, Pedro Henrique
    Scodro, Regiane B. L.
    Nakamura, Celso V.
    Leite, Clarice Queico F.
    Siqueira, Vera Lucia D.
    Cardoso, Rosilene F.
    TUBERCULOSIS, 2016, 97 : 65 - 72
12345