Mechanical vibrations increase the proliferation of articular chondrocytes in high-density culture

被引:24
|
作者
Kaupp, J. A. [1 ,2 ]
Waldman, S. D. [1 ,2 ,3 ]
机构
[1] Queens Univ, Dept Mech & Mat Engn, Kingston, ON K7L 3N6, Canada
[2] Kingston Gen Hosp, Human Mobil Res Ctr, Kingston, ON, Canada
[3] Queens Univ, Dept Chem Engn, Kingston, ON K7L 3N6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
articular cartilage; chondrocytes; tissue engineering; mechanical stimulation; vibration; proliferation;
D O I
10.1243/09544119JEIM376
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Tissue engineering is a promising approach for articular cartilage repair; however, it still has proven a challenge to produce tissue from the limited number of cells that can be extracted from a single individual. Relatively few cell expansion methods exist without the problems of dedifferentiation and/or loss of potency. Previously, it has been shown that mechanical vibrations can enhance chondrocyte proliferation in monolayer culture. Thus, it was hypothesized that chondrocytes grown in high-density Culture would respond in a similar fashion while maintaining phenotypic stability. Isolated bovine articular chondrocytes were seeded in high-density culture on Millicell (TM) filters and Subjected to mechanical vibrations 48 h after seeding. Mechanical vibrations enhanced chondrocyte proliferation at frequencies above 350 Hz, with the peak response occurring at a 1g amplitude for a duration of 30 min. Under these conditions, the gene expression of cartilage-specific and dedifferentiation markers (collagen 11, collagen 1, and aggrecan) were unchanged by the imposed stimulus. To determine the effect of accumulated extracellular matrix (ECM) on this proliferative response, selected Cultures were Stimulated under the same conditions after varying lengths of preculture. The amount of accumulated ECM (collagen and proteoglycans) decreased this proliferative response, with the Cultures becoming insensitive to the stimulus after I week of preculture. Thus, mechanical vibration can serve as an effective means preferentially to Stimulate the proliferation of chondrocytes during culture, but its effects appear to be limited to the early stages where ECM accumulation is at a minimum.
引用
收藏
页码:695 / 703
页数:9
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