Characterization of a cfr-positive methicillin-resistant Staphylococcus epidermidis strain of the lineage ST22 implicated in a life-threatening human infection

被引：20

作者：

Lozano, Carmen ^{[1
]}

Ruiz-Garcia, Montserrat ^{[2
]}

Gomez-Sanz, Elena ^{[1
]}

Lopez-Garcia, Pilar ^{[2
]}

Royo-Garcia, Gloria ^{[2
]}

Zarazaga, Myriam ^{[1
]}

Torres, Carmen ^{[1
]}

机构：

[1] Univ La Rioja, Area Bioquim & Biol Mol, Logrono 26006, Spain

[2] Hosp Gen Univ Elche, Secc Microbiol, Elche 03203, Spain

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2012年 / 73卷 / 04期

关键词：

cfr; MRSE; ST22; fexA; Linezolid resistance; 23S RIBOSOMAL-RNA; LINEZOLID RESISTANCE; UNITED-STATES; 1ST REPORT; GENE CFR; AUREUS; CHLORAMPHENICOL; FLORFENICOL; MUTATIONS; MECHANISM;

D O I：

10.1016/j.diagmicrobio.2012.04.013

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The cfr gene was identified in 3 methicillin-resistant Staphylococcus epidermidis (MRSE) isolates of lineage ST22 implicated in a fatal human infection. MRSE isolates displayed an indistinguishable pulsed-field gel electrophoresis profile and SCCmec type III, and showed a multiresistance phenotype. The presence of cfr, fexA, aac(6')-aph(2 ''), and dfrS1 genes was confirmed by polymerase chain reaction and sequencing. A mutation in 23S rRNA gene (C2534T) and amino acid changes and/or insertions in 13 and L4 proteins were detected. The cfr and fexA genes were located in a conjugative plasmid of approximately 45 kb and in the chromosome. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：380 / 382

页数：3

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