Direct Visualization of the Movement of a Single T7 RNA Polymerase and Transcription on a DNA Nanostructure

被引：30

作者：

Endo, Masayuki ^{[1
,3
]}

Tatsumi, Koichi ^{[2
]}

Terushima, Kosuke ^{[2
]}

Katsuda, Yousuke ^{[2
]}

Hidaka, Kumi ^{[2
]}

Harada, Yoshie ^{[1
]}

Sugiyama, Hiroshi ^{[1
,2
,3
]}

机构：

[1] Kyoto Univ, Inst Integrated Cell Mat Sci WPI iCeMS, Sakyo Ku, Kyoto 6068501, Japan

[2] Kyoto Univ, Dept Chem, Grad Sch Sci, Sakyo Ku, Kyoto 6068502, Japan

[3] Japan Sci & Technol Corp JST, CREST, Chiyoda Ku, Tokyo 1020075, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 35期

关键词：

DNA origami; high-speed AFM; nanostructures; single-molecule experiments; transcription; ATOMIC-FORCE MICROSCOPY; MOLECULE ANALYSIS; NANOSCALE SHAPES; FOLDING DNA; DIFFUSION; COMPLEXES; ORIGAMI;

D O I：

10.1002/anie.201201890

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Snapshots of transcription: Movement of a single molecule of T7 RNA polymerase (RNAP) along a double-stranded DNA (dsDNA) template attached to a DNA origami platform (green, see scheme) was recorded using high-speed AFM. A one kilobase template dsDNA containing the T7 promoter was used to record AFM images of transcription of the template dsDNA by RNAP and the resulting biotinylated RNA product was detected by streptavidin-labeling. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：8778 / 8782

页数：5

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