Comparison of two commercial real-time reverse transcriptase polymerase chain reaction assays for porcine reproductive and respiratory syndrome virus

被引：0

作者：

Harmon, Karen M. ^{[1
]}

Abate, Sarah A. ^{[1
]}

Chriswell, Amy J. ^{[1
]}

Chittick, Wayne A. ^{[1
]}

Strait, Erin L. ^{[1
]}

机构：

[1] Iowa State Univ, Coll Vet Med, Vet Diagnost Lab, Ames, IA 50011 USA

来源：

JOURNAL OF SWINE HEALTH AND PRODUCTION | 2012年 / 20卷 / 04期

关键词：

PCR;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Specimens were tested for porcine reproductive and respiratory syndrome virus by real-time reverse transcriptase polymerase chain reaction (RT-PCR) with two commercial agent-specific assays. Result discrepancy between the two tests was confirmed by conventional RT-PCR, sequencing, or both on six of 423 clinical cases. Neither assay detected every positive case.

引用

页码：184 / 188

页数：5

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