Comparison of two commercial real-time reverse transcriptase polymerase chain reaction assays for porcine reproductive and respiratory syndrome virus

被引：0
作者：
Harmon, Karen M. [1 ]
Abate, Sarah A. [1 ]
Chriswell, Amy J. [1 ]
Chittick, Wayne A. [1 ]
Strait, Erin L. [1 ]
机构：
[1] Iowa State Univ, Coll Vet Med, Vet Diagnost Lab, Ames, IA 50011 USA
来源：
JOURNAL OF SWINE HEALTH AND PRODUCTION | 2012年 / 20卷 / 04期
关键词：
PCR;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Specimens were tested for porcine reproductive and respiratory syndrome virus by real-time reverse transcriptase polymerase chain reaction (RT-PCR) with two commercial agent-specific assays. Result discrepancy between the two tests was confirmed by conventional RT-PCR, sequencing, or both on six of 423 clinical cases. Neither assay detected every positive case.
引用
收藏
页码：184 / 188
页数：5
相关论文
共 8 条
  • [1] AHL R, 1992, VET REC, V130, P87
  • [2] Evolution of porcine reproductive and respiratory syndrome virus during sequential passages in pigs
    Chang, CC
    Yoon, KJ
    Zimmerman, JJ
    Harmon, KM
    Dixon, PM
    Dvorak, CMT
    Murtaugh, MP
    [J]. JOURNAL OF VIROLOGY, 2002, 76 (10) : 4750 - 4763
  • [3] Comparison of RNA extraction and real-time reverse transcription polymerase chain reaction methods for the detection of Porcine reproductive and respiratory syndrome virus in porcine oral fluid specimens
    Chittick, Wayne A.
    Stensland, Wendy R.
    Prickett, John R.
    Strait, Erin L.
    Harmon, Karen
    Yoon, Kyoung-Jin
    Wang, Chong
    Zimmerman, Jeffrey J.
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2011, 23 (02) : 248 - 253
  • [4] DETECTION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS IN BOAR SEMEN BY PCR
    CHRISTOPHERHENNINGS, J
    NELSON, EA
    NELSON, JK
    HINES, RJ
    SWENSON, SL
    HILL, HT
    ZIMMERMAN, JJ
    KATZ, JB
    YAEGER, MJ
    CHASE, CCL
    BENFIELD, DA
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (07) : 1730 - 1734
  • [5] The impact of primer and probe-template mismatches on the sensitivity of pandemic influenza A/H1N1/2009 virus detection by real-time RT-PCR
    Klungthong, Chonticha
    Chinnawirotpisan, Piyawan
    Hussem, Kittinun
    Phonpakobsin, Thipwipha
    Manasatienkij, Wudtichai
    Ajariyakhajorn, Chuanpis
    Rungrojcharoenkit, Kamonthip
    Gibbons, Robert V.
    Jarman, Richard G.
    [J]. JOURNAL OF CLINICAL VIROLOGY, 2010, 48 (02) : 91 - 95
  • [6] SYBR green-based real-time quantitative PCR assay for detection of West Nile virus circumvents false-negative results due to strain variability
    Papin, JF
    Vahrson, W
    Dittmer, DP
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (04) : 1511 - 1518
  • [7] Ratcliff RM, 2007, CURR ISSUES MOL BIOL, V9, P87
  • [8] Evaluation of the sensitivity of reverse-transcription polymerase chain reaction to detect porcine reproductive and respiratory syndrome virus on individual and pooled samples from boars
    Rovira, Albert
    Clement, Travis
    Christopher-Hennings, Jane
    Thompson, Bob
    Engle, Mark
    Reicks, Darwin
    Munoz-Zanzi, Claudia
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2007, 19 (05) : 502 - 509
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